Choline deficiency decreased the growth performances and damaged the amino acid absorption capacity in juvenile grass carp (Ctenopharyngodon idella)
Choline deficiency decreased the growth performances and damaged the amino acid absorption capacity in juvenile grass carp (Ctenopharyngodon idella)
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胆碱缺乏会降低草鱼幼鱼的生长性能并损害氨基酸吸收能力
DOI：
10.1016/j.aquaculture.2019.734829
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发表时间：
2020-03
期刊：
影响因子：
4.5
通讯作者：
Xiaoqiu Zhou
中科院分区：
文献类型：
--
作者：
Zehong Yuan;Lin Feng;Weidan Jiang;Pei Wu;Yang Liu;Jun Jiang;Shengyao Kuang;Ling Tang;Xiaoqiu Zhou
研究了胆碱对草鱼幼鱼生长、肠道组织学、肠道氨基酸吸收能力和信号传导的影响。鱼被喂食六个等级的胆碱（142.2，407.4，821.6，1215.8，1589.3，1996.6 mg kg−1）70天。结果表明，胆碱缺乏会影响生长性能，并损害鱼类肠道健康。值得注意的是，胆碱通过非均匀的机制影响不同肠段对各种AA的吸收能力。首先，胆碱缺乏导致所有肠段中Met、Trp、Thr、Ile、Leu、瓦尔、Gly、Pro、Ser、Cys和Tau等游离中性AA含量降低，部分原因是相应转运蛋白SLC 7A 7、SLC 7A 6、SLC 1A 5、SLC 7A 9的mRNA水平降低（除了在MI中）、SLC7A8、SLC6A6、SLC6A19b、SLC38A2（除了在PI中）和肽转运蛋白1（SLC15A1）以及SLC1A5在肠中的蛋白水平。其次，胆碱缺乏降低了三个肠段中游离碱性氨基酸（赖氨酸、精氨酸和组氨酸）的含量（DI除外），这部分与肠段中阳离子型AAT SLC7A1和SLC15A1 mRNA水平以及SLC7A1蛋白水平的下调有关。第三，胆碱缺乏降低了三个肠段中Glu和Asp等游离酸性氨基酸的含量，这部分与相应的转运蛋白SLC1A2a和SLC15A1的mRNA水平降低有关。胆碱不足抑制TOR（雷帕霉素的目标）信号分子mRNA水平和TOR蛋白丰度在鱼肠道。此外，根据PWG（增重百分比）和FE（饲料效率）计算草鱼幼鱼的胆碱需要量分别为1330.7和1283.4 mg kg− 1饲料。总的来说，胆碱缺乏会减弱草鱼幼鱼肠道氨基酸的吸收能力，这可能与肠道健康状况的恶化有关，氨基酸转运蛋白的减少可能与TOR信号通路的抑制有关。
The present study investigated the effects of choline on growth, intestinal histology, intestinal amino acid (AA) absorption capacity and the underlying signalling in juvenile grass carp (Ctenopharyngodon idella). Fish were fed six-graded levels of choline (142.2, 407.4, 821.6, 1215.8, 1589.3, 1996.6 mg kg−1) for 70 days. The results showed that choline insufficiency deteriorated the growth performance and injured the fish intestinal health. Strikingly, choline influenced the absorptive capacity for various sorts of AAs in different intestinal segments by non-uniform mechanisms. Firstly, choline deficiency reduced the contents of free neutral AAs including Met, Trp, Thr, Ile, Leu, Val, Gly, Pro, Ser, Cys and Tau in all intestinal segments partly attributed to decrease the mRNA levels of the corresponding transporters including SLC7A7, SLC7A6, SLC1A5, SLC7A9 (except in MI), SLC7A8, SLC6A6, SLC6A19b, SLC38A2 (except in PI) and peptide transporter 1 (SLC15A1) as well as the protein level of SLC1A5 in the intestine. Secondly, choline deficiency decreased the contents of free basic AAs including Lys, Arg, and His (except in DI) in three segments partly associated with downregulating cationic AAT SLC7A1 and SLC15A1 mRNA levels as well as the protein level of SLC7A1 in gut segments. Thirdly, choline deficiency decreased the contents of free acidic AAs including Glu and Asp in three gut segments partly associated with the decreasing mRNA levels of the corresponding transporters including SLC1A2a and SLC15A1 in the intestine. Choline insufficiency depressed the TOR (target of rapamycin) signalling molecule mRNA levels and TOR protein abundance in the fish gut. In addition, choline requirement of juvenile grass carp was calculated to be 1330.7 and 1283.4 mg kg−1diet based on the PWG (percent weight gain) and FE (feed efficiency), respectively. In general, choline deficiency attenuated the absorptive abilities of intestinal AAs in juvenile grass carp, which might be connected with the aggravation of intestinal health, the decrease of amino acid transporters might be related to restraint the TOR signalling pathway.
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影响因子：
3.7
作者：
Zhao HF;Feng L;Jiang WD;Liu Y;Jiang J;Wu P;Zhao J;Kuang SY;Tang L;Tang WN;Zhang YA;Zhou XQ
通讯作者：
Zhou XQ
影响因子：
4.7
作者：
Jing Xu;Pei Wu;Wei‐dan Jiang;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Wuneng Tang;Yong‐An Zhang-Yong‐
通讯作者：
Jing Xu;Pei Wu;Wei‐dan Jiang;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Wuneng Tang;Yong‐An Zhang-Yong‐
DOI：
10.5713/ajas.14.0532
发表时间：
2015-05
期刊：
Asian-Australasian journal of animal sciences
影响因子：
--
作者：
Khosravi S;Jang JW;Rahimnejad S;Song JW;Lee KJ
通讯作者：
Lee KJ
影响因子：
3.7
作者：
Chandler TL;White HM
通讯作者：
White HM
DOI：
10.1042/cs20150554
发表时间：
2016-04-01
期刊：
Clinical science (London, England : 1979)
影响因子：
--
作者：
Rosario FJ;Dimasuay KG;Kanai Y;Powell TL;Jansson T
通讯作者：
Jansson T