Impact of lime, nitrogen and plant species on fungal community structure in grassland microcosms
Impact of lime, nitrogen and plant species on fungal community structure in grassland microcosms
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DOI：
10.1111/j.1462-2920.2005.00748.x
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Clipson, N
中科院分区：
文献类型：
--
作者：
Kennedy, N;Connolly, J;Clipson, N
采用基于微观世界的方法研究植物和化学因素对高地酸性草原土壤真菌群落结构的影响。未改良和施肥草地上的七种典型植物要么不进行改良，要么用石灰、氮或石灰加氮进行处理。通过分子方法、真菌自动核糖体基因间间隔区分析（FARISA）评估真菌群落结构，同时通过测量土壤麦角甾醇含量估计真菌生物量。添加氮（有或没有石灰）的效果最大，降低了土壤 pH 值、真菌生物量和真菌核糖型数量，但真菌群落结构几乎没有相应的变化。尽管不同植物物种与真菌生物量的一些变化有关，但这并没有导致植物物种之间真菌群落结构的显着差异。单独添加石灰不会引起真菌生物量、核糖型数量或群落结构的变化。总体而言，真菌群落结构似乎受到植物物种和化学处理之间相互作用的更显着影响，而不是直接受到个体改良因素变化的影响。这些结果与相同土壤的细菌群落的结果形成鲜明对比，后者因化学物质（石灰和氮）的添加而发生了很大的变化。
A microcosm-based approach was used to study impacts of plant and chemical factors on the fungal community structure of an upland acidic grassland soil. Seven plant species typical of both unimproved and fertilized grasslands were either left unamended or treated with lime, nitrogen or lime plus nitrogen. Fungal community structure was assessed by a molecular approach, fungal automated ribosomal intergenic spacer analysis (FARISA), while fungal biomass was estimated by measuring soil ergosterol content. Addition of nitrogen (with or without lime) had the largest effect, decreasing soil pH, fungal biomass and fungal ribotype number, but there was little corresponding change in fungal community structure. Although different plant species were associated with some changes in fungal biomass, this did not result in significant differences in fungal community structure between plant species. Addition of lime alone caused no changes in fungal biomass, ribotype number or community structure. Overall, fungal community structure appeared to be more significantly affected through interactions between plant species and chemical treatments, as opposed to being directly affected by changes in individual improvement factors. These results were in contrast to those found for the bacterial communities of the same soils, which changed substantially in response to chemical (lime and nitrogen) additions.