Successfully controlled isomerization and pheophytinization of bacteriochlorophyll b by weak acid in the dark in vitro
Successfully controlled isomerization and pheophytinization of bacteriochlorophyll b by weak acid in the dark in vitro
复制标题
体外弱酸成功控制细菌叶绿素b的异构化和脱镁叶绿素化
DOI：
10.1016/s0003-2670(98)00030-0
复制
发表时间：
1998
影响因子：
6.2
通讯作者：
H. Kise
中科院分区：
文献类型：
--
作者：
Masami Kobayashi;M. Yamamura;Satoshi Akutsu;J. Miyake;M. Hara;M. Akiyama;H. Kise
在黑暗中，细菌叶绿素b(BCHLb)丙酮溶液中加入酸后，795 nm处的红外吸收峰迅速消失，675 nm处的吸光度同时增大。这些光学变化是光不依赖的异构化反应的结果，该异构化反应将Bchl b转化为3-去乙烯基-3-乙酰基叶绿素a。然而，在丙酮水溶液中，Bchl b在酸的存在下发生脱金属反应，生成细菌叶绿素b。Bchl b的异构化反应和脱金属反应均在黑暗中被酸成功地控制。
The infrared absorbance peak at 795nm rapidly disappears while the absorbance at 675nm increases simultaneously with the addition of acid to a solution of bacteriochlorophyll b (BChl b) in acetone in the dark. These optical changes result from light-independent isomerization, which converts BChl b to 3-desvinyl-3-acetyl-chlorophyll a. However, in aqueous acetone in the presence of acid, BChl b undergoes pheophytinization (demetalation) to yield bacteriopheophytin b. Both isomerization and pheophytinization of BChl b were successfully controlled by acid in the dark.