Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked
Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked
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DOI：
10.1007/s10856-008-3627-7
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发表时间：
2009-02-01
影响因子：
3.7
通讯作者：
Mansur, Herman S.
中科院分区：
文献类型：
--
作者：
Costa, Ezequiel de Souza, Jr.;Pereira, Marivalda M.;Mansur, Herman S.
在目前的工作中，我们报告的合成，表征，和初步的生物相容性的聚合物共混物的基础上，壳聚糖和聚乙烯醇（PVA）与低水解度和化学交联的戊二醛在皮肤组织修复的潜在应用。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM/EDX）对共混水凝胶的微观结构和形貌进行了表征。通过溶胀作为使用模拟体液的初步体外测试来研究水凝胶。此外，生物相容性，细胞毒性和细胞活力进行了评估，通过MTT法与VERO细胞培养和细胞铺展粘附分析。结果发现，通过增加壳聚糖与PVA的比例，材料的模拟体液吸收显著改变。所有测试的水凝胶都清楚地呈现出足够的细胞活力、无毒性和合适的性质，这些性质可以定制用于皮肤组织工程的预期用途。
In the present work we report the synthesis, characterization, and preliminary biocompatibility of polymer blends based on Chitosan and poly(vinyl alcohol) (PVA) with low degree of hydrolysis and chemically crosslinked by glutaraldehyde for potential application on skin tissue repairing. The microstructure and morphology of the blended hydrogels were characterized through Fourier Transform Infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM/EDX) analysis. Hydrogels were investigated by swelling as preliminary in vitro test using simulated body fluid. In addition, biocompatibility, cytotoxicity, and cell viability were assessed via MTT assay with VERO cell culture and cell spreading-adhesion analysis. It was found that by increasing the chitosan to PVA ratio, simulated body fluid uptake of the material was significantly altered. All the tested hydrogels have clearly presented adequate cell viability, non-toxicity, and suitable properties which can be tailored for prospective use in skin tissue engineering.