Dietary magnesium deficiency in rats enhances free radical production in skeletal muscle.
Dietary magnesium deficiency in rats enhances free radical production in skeletal muscle.
复制标题
大鼠膳食镁缺乏会增强骨骼肌中自由基的产生。
DOI：
10.1093/jn/125.5.1205
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Yves Rayssiguier
中科院分区：
文献类型：
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作者：
Edmond Rock;Catherine Astier;C. Lab;Xavier Vignon;E. Gueux;C. Motta;Yves Rayssiguier
最近的研究表明，自由基可能参与镁缺乏引起的组织损伤。本研究的目的是评估镁缺乏对骨骼肌组织自由基产生的影响。雄性Wistar大鼠从断奶开始配对喂养12天，对照组或含960或40 mg镁/kg饮食的镁缺乏饮食。在镁缺乏大鼠，低镁血症伴随着显着降低镁和更高的钙浓度在骨骼肌组织。骨骼肌组织的电子显微镜显示超微结构的变化，包括肿胀的线粒体和肌浆网的解体。使用自旋捕获技术，我们表明，显着更多的羟基自由基产生在肌肉匀浆中的镁缺乏大鼠。此外，在两组中外源性铁的存在下，自旋陷阱加合物的量增加。与这些观察结果相一致，与对照组相比，在镁缺乏组的骨骼肌中发现了更高浓度的硫代巴比妥酸反应物质和更低浓度的巯基。这些结果有力地支持了这一假设，即自由基介导的损伤可能有助于骨骼肌病变引起的镁缺乏。
Recent studies suggest that free radicals may be involved in tissue injuries induced by magnesium deficiency. The aim of the present study was to assess the effect of magnesium deficiency on free radical production of skeletal muscle tissue. Male Wistar rats were pair-fed from weaning for 12 d either control or Mg-deficient diets containing 960 or 40 mg magnesium/kg diet, respectively. In the Mg-deficient rats, hypomagnesemia was accompanied by significantly lower magnesium and greater calcium concentrations in skeletal muscle tissue. Electron microscopy of skeletal muscle tissue revealed ultrastructural changes, including swelling mitochondria and disorganization of the sarcoplasmic reticulum network. Using the spin-trapping technique, we showed that significantly more hydroxyl radicals were generated in muscle homogenates of Mg-deficient rats. Moreover, the amount of spin trap adducts was increased in the presence of exogenous iron in both groups. In agreement with these observations, a greater concentration of thiobarbituric acid-reactive substances and a lower concentration of thiol groups were found in skeletal muscle of the Mg-deficient group compared with controls. These results strongly support the hypothesis that free radical-mediated injury could contribute to skeletal muscle lesions resulting from magnesium deficiency.