The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells.
The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells.
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小鼠 IL 2 受体。
DOI：
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发表时间：
1984
影响因子：
4.4
通讯作者：
Thomas R. Malek
中科院分区：
文献类型：
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作者：
G. Ortega;R. Robb;Ethan M. Shevach;Thomas R. Malek
对小鼠IL - 2受体的三种不同的大鼠单克隆抗体3C7、7D4和2E4的特性进行了结合、生化和功能分析比较。3C7似乎定义了一个接近或相同于受体IL - 2结合位点的表位，因为3C7抑制了放射性标记的IL - 2与CTL-L细胞的结合，因为未标记的IL - 2抑制了FITC-3C7与CTL-L细胞的结合。7D4和2E4对IL - 2结合无影响。竞争性抗体结合研究证实，3C7观察到的表位与7D4和2E4观察到的表位不同。序列免疫沉淀研究表明，所有三种抗体都对相同的分子种类有反应，并且每种抗体都从CTL-L表面沉淀20,000至25,000道尔顿，50,000至60,000道尔顿和100,000至120,000道尔顿的相同成分。FACS研究证明了每个抗体定义的抗原在数量和质量上相同的细胞分布。它们不能染色超过95%的静息淋巴细胞，但对Con A T母细胞反应强烈，对LPS B母细胞反应较弱。未标记的IL - 2也能抑制FITC-3C7与LPS B细胞原细胞的结合，表明活化的B细胞上存在IL - 2结合位点。每种抗体均抑制IL - 2驱动的HT2或CTL-L细胞增殖。3C7和7D4比2E4具有更强的增殖抑制作用，并且与单独使用任何一种抗体相比，3C7和7D4联合使用对增殖的抑制水平更高。总的来说，结果支持了这些抗体检测IL - 2受体的两个不同功能区域的假设。
The properties of three distinct rat monoclonal antibodies, designated 3C7, 7D4, and 2E4, to the murine IL 2 receptor have been compared in binding, biochemical, and functional assays. 3C7 appears to define an epitope near or identical to the IL 2-binding site of the receptor, because 3C7 inhibited the binding of radiolabeled IL 2 to CTL-L cells and because unlabeled IL 2 inhibited the binding of FITC-3C7 to CTL-L cells. 7D4 and 2E4 had no effect on IL 2 binding. Competitive antibody-binding studies confirmed that the epitope seen by 3C7 was distinct from the epitope(s) seen by 7D4 and 2E4. Sequential immunoprecipitation studies demonstrated that all three antibodies were reactive with the same molecular species, and that each precipitated identical components of 20,000 to 25,000 daltons, 50,000 to 60,000 daltons, and 100,000 to 120,000 daltons from the surface of CTL-L. FACS studies demonstrated a quantitatively and qualitatively identical cell distribution for the antigen defined by each antibody. They failed to stain more than 95% of resting lymphocytes, but were strongly reactive with Con A T blasts and substantially less reactive with LPS B blasts. Unlabeled IL 2 was also able to inhibit the binding of FITC-3C7 to LPS B cell blasts, suggesting the presence of IL 2-binding sites on activated B cells. Each antibody inhibited IL 2-driven proliferation of HT2 or CTL-L cells. 3C7 and 7D4 were more potent inhibitors of proliferation than was 2E4, and the combined use of 3C7 and 7D4 resulted in greater levels of inhibition of proliferation than that shown from the use of either antibody alone. Collectively, the results support the hypothesis that these antibodies detect two distinct functional regions of the IL 2 receptor.