Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles.
Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles.
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DOI：
10.1007/978-1-4939-6848-0_7
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhang P
中科院分区：
文献类型：
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作者：
Lee C;Wang P;Gaston MA;Weiss AA;Zhang P
利用金纳米粒子的等离子体峰移现象和血凝素对病毒和唾液酸对动物细胞的感染机制，研制了检测病毒的生物传感器。胶体金纳米颗粒的等离子体激元峰随着颗粒之间的等离子体激元相互作用而发生变化，胶体金纳米颗粒溶液的颜色从酒红色变为紫色。由于血凝素-唾液酸相互作用，通过在溶液中添加病毒颗粒诱导唾液酸减少和稳定的胶体金纳米颗粒聚集。本工作采用简单的一锅法、绿色法，在不对唾液酸进行化学修饰的情况下，合成了唾液酸还原并稳定的金纳米粒子（d = 20.1 ± 1.8 nm）。金纳米粒子显示出通过血凝素-唾液酸结合与病毒颗粒的靶向特异性聚集。在化学灭活的B型流感病毒种的光密度变化和稀释度之间观察到线性相关性。病毒稀释液的检测限（血凝试验滴度，512）显示为0.156体积%，使用更多的唾液酸-金纳米颗粒可以扩展线性上限。
Biosensor for the detection of virus was developed by utilizing plasmonic peak shift phenomenon of the gold nanoparticles and viral infection mechanism of hemagglutinin on virus and sialic acid on animal cells. The plasmonic peak of the colloidal gold nanoparticles changes with the aggregation of the particles due to the plasmonic interaction between nearby particles and the color of the colloidal nanoparticle solution changes from wine red to purple. Sialic acid reduced and stabilized colloidal gold nanoparticle aggregation is induced by the addition of viral particles in the solution due to the hemagglutinin-sialic acid interaction. In this work, sialic acid reduced and stabilized gold nanoparticles (d = 20.1 ± 1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B virus species. The detection limit of the virus dilution (hemagglutinination assay titer, 512) was shown to be 0.156 vol% and the upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.