GENOMIC AND EST MICROSATELLITES FOR RHODODENDRON AUREUM (ERICACEAE) AND CROSS-AMPLIFICATION IN OTHER CONGENERIC SPECIES
GENOMIC AND EST MICROSATELLITES FOR RHODODENDRON AUREUM (ERICACEAE) AND CROSS-AMPLIFICATION IN OTHER CONGENERIC SPECIES
复制标题
杜鹃花（杜鹃花科）的基因组和 EST 微卫星以及其他同属物种的交叉扩增
DOI：
10.3732/ajb.1100153
复制
发表时间：
2011-09-01
影响因子：
3
通讯作者：
Xiao, Hong-Xing
中科院分区：
文献类型：
--
作者：
Li, Lin-Feng;Yin, Dong-Xu;Xiao, Hong-Xing
本研究的目的：本研究的目的是开发濒危植物金色杜鹃的基因组和表达序列标签（EST）衍生的微卫星，并评估其在杜鹃中的可转移性。dauricum和R.方法和结果：在短果红树莓中分离到12个基因组微卫星。利用FIASCO技术对10个杜鹃花属植物的10个微卫星序列进行了分析，并利用GenBank中杜鹃花dbEST数据库对7个EST来源的微卫星进行了分析。每个位点的等位基因数从1到8不等。观察杂合度和期望杂合度分别为0.00 ~ 1.00和0.00 ~ 0.73。金色其中16个和10个基因座在红曲霉中扩增成功。brachycarpum和R. dauricum）。这些微卫星标记在遗传多样性和保护遗传学研究中具有潜在的应用价值。
Premise of the study: Our objective of this study was to develop genomic and expressed sequence tag (EST)-derived microsatellites for the endangered species Rhododendron aureum and access their transferability in R. dauricum and R. brachycarpum.Methods and Results: Twelve genomic microsatellites were isolated in R. aureum using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol and seven EST-derived microsatellites were characterized by screening the Rhododendron dbEST database of GenBank. The number of alleles per locus ranged from one to eight. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.00 to 0.73, respectively.Conclusions: A total of 19 microsatellite loci were developed for R. aureum. Sixteen and 10 of these loci were successfully amplified in R. brachycarpum and R. dauricum, respectively. These microsatellite markers will have potential applications in genetic diversity and conservation genetics studies.