Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea.
Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea.
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电泳检测乙基亚硝基脲在小鼠中诱导的生发突变。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
Susan E. LEWISt
中科院分区：
文献类型：
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作者：
F. M. Johnson;Susan E. LEWISt
雄性小鼠DBA/2J和C57BL/6J以250 mg/kg体重的剂量注射乙基亚硝基脲。在11周的不育期后，接受治疗的动物与未接受治疗的雌性交配；C57治疗DBA， DBA治疗C57。建立了一个未经治疗的雄性小对照组，并从早期实验中建立了一个较大的对照组进行比较。从父母和F1后代手术切除的组织样本进行电泳检查。在22512个突变检测位点中发现了9个可归因于EtNU诱导的新突变体。290,252个对照基因座试验未发现突变。一项与显性致死效应和物理畸形的电泳分析同时进行的初步研究显示，由于EtNU处理精原细胞，在背景上没有增加，这表明EtNU诱导的突变倾向于不以这种方式表达。通过电泳鉴定的突变体的性质有助于后续分析，从而增加对哺乳动物生物体中诱导突变的分子基础和生理效应的理解。
Male DBA/2J and C57BL/6J mice were treated with ethylnitrosourea (EtNU) at a dose of 250 mg/kg of body weight. After a sterile period of 11 weeks, the treated animals were mated with untreated females; treated DBA with C57 and treated C57 with DBA. A small control group of untreated males was established, and a larger control group from earlier experiments was also used for comparison. Tissue samples removed surgically from the parents and F1 offspring were examined by electrophoresis. Nine newly arisen mutants attributable to induction by EtNU were found in 22,512 loci tested for mutation. No mutations were encountered in 290,252 control locus tests. A preliminary study conducted concomitantly with the electrophoretic analyses for dominant lethal effects and physical malformation showed no increase over background due to EtNU treatment of spermatogonia, which suggests that mutations induced by EtNU tend not to be expressed in this way. The nature of the mutants identified by electrophoresis facilitates follow-up analyses leading to increased understanding of the molecular basis and the physiological effects of induced mutations in mammalian organisms.