Nuclear Magnetic Resonance Microscopy of Atheroma in Human Coronary Arteries
Nuclear Magnetic Resonance Microscopy of Atheroma in Human Coronary Arteries
复制标题
人类冠状动脉粥样斑块的核磁共振显微镜检查
DOI：
10.1177/000331979104200906
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发表时间：
1991
期刊：
影响因子：
2.8
通讯作者：
J. Ackerman
中科院分区：
文献类型：
--
作者：
J. Pearlman;J. Southern;J. Ackerman
本研究的目的是使用直接核磁共振（NMR）显微镜定量和图像的人类冠状动脉粥样硬化脂质的积累，并通过与组织学制剂的比较，以验证结果。NMR显微术在超导实验NMR成像系统上进行，该系统在2特斯拉下操作，具有设计用于短回波时间（TE）、强B 1场强度和小样品的探针。数据采集使用多偏移化学编码，偏移量基于人体动脉血管壁内粥样硬化脂质的热致光谱特征。三个独立的图像数据通道产生血管壁内粥样硬化的彩色轴显示。组织学上的动脉粥样硬化位置通过间质的稀疏来确定，因为脂质在石蜡包埋过程中被提取出来。测定管腔、动脉粥样硬化和外壁的周长、面积和形状指数（周长2：4π面积），并将NMR与组织学进行比较。除管腔形状指数外，其他测量值无显著差异，组织学检查显示管腔形状指数均匀较大，这归因于组织学制备期间血管变平。冠状动脉壁粥样硬化含量的NMR测量与组织学一致（r = 0.996）。具有彩色轴显示的NMR显微镜被证明能够定量和成像冠状动脉中的粥样硬化，避免了与固定、包埋和切片组织学相关的扭曲和脂质去除。
The purpose of this study was to use direct nuclear magnetic resonance (NMR) microscopy to quantitate and image accumulations of atheroma lipids in human coronary arteries and to validate the results by comparison with histologic preparations. NMR microscopy was performed on a superconducting experimental NMR imaging system operating at 2 Tesla with a probe designed for short echo time (TE), strong B 1 field strength, and small samples. Data acquisition used multiple-offset chemical encoding with offsets based on the thermotropic spectral signature of atheroma lipids within the human arterial vessel wall. Three separate channels of image data yielded color axis display of atheroma within the vessel walls. Atheroma location by histology was identified by rarefaction of stroma, as the lipids are extracted in the process of embedding in paraffin. Perimeters, areas, and a shape index (perimeter2:4πarea) of lumen, atheroma, and outer wall were determined and compared for NMR vs histology. There was no significant difference in the measurements with the exception of luminal shape indices, which were uniformly larger by histology, attributable to flattening of the vessels during histologic preparation. NMR measurement of atheroma content of coronary artery walls agreed well with histology (r = 0.996). NMR microscopy with color axis display proved able to quantitate and image atheroma in coronary arteries, obviating the distortions and lipid removal associated with fixation, embedding, and sectioning for histology.