Orphan nuclear receptor βFTZ-F1 is required for muscle-driven morphogenetic events at the prepupal-pupal transition in Drosophila melanogaster
Orphan nuclear receptor βFTZ-F1 is required for muscle-driven morphogenetic events at the prepupal-pupal transition in Drosophila melanogaster
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DOI：
10.1016/s0012-1606(03)00036-8
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发表时间：
2003-05-01
影响因子：
2.7
通讯作者：
Woodard, CT
中科院分区：
文献类型：
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作者：
Fortier, TA;Vasa, PP;Woodard, CT
在果蝇中，20-羟基蜕皮激素（ecdysone）滴度的波动协调变态过程中的基因表达、细胞死亡和形态发生。我们之前的研究支持这样的假设：betaFTZ-F1（一种孤儿核受体）为特定基因提供了在适当时间被蜕皮激素诱导的能力，从而指导了前蛹-蛹转变的关键发育事件。我们正在研究 betaFTZ-F1 在形态发生中的作用。我们对对照动物和 betaF7Z-F1 突变动物变态过程中的形态发生事件进行了详细研究。我们发现 betaFTZ-F1 突变体的腿部发育正常进行，直到预蛹-蛹过渡，此时最终的腿部伸长延迟了几个小时，并且在突变体中显着减少。我们还表明 betaFTZ-F1 突变体在预蛹-蛹转变时无法完全伸展翅膀并缩短身体。我们发现 betaFTZ-F1 突变体无法正确执行驱动这些过程的肌肉收缩。通过在蛹前-蛹过渡的正常时间内使突变体承受压力下降，可以挽救一些缺陷。我们的研究结果表明，βFTZ-F1 指导果蝇前蛹-蛹转变期间驱动主要形态发生过程的肌肉收缩事件。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
In Drosophila melanogaster, fluctuations in 20-hydroxyecdysone (ecdysone) titer coordinate gene expression, cell death, and morphogenesis during metamorphosis. Our previous studies have supported the hypothesis that betaFTZ-F1 (an orphan nuclear receptor) provides specific genes with the competence to be induced by ecdysone at the appropriate time, thus directing key developmental events at the prepupal-pupal transition. We are examining the role of betaFTZ-F1 in morphogenesis. We have made a detailed study of morphogenetic events during metamorphosis in control and betaF7Z-F1 mutant animals. We show that leg development in betaFTZ-F1 mutants proceeds normally until the prepupal-pupal transition, when final leg elongation is delayed by several hours and significantly reduced in the mutants. We also show that betaFTZ-F1 mutants fail to fully extend their wings and to shorten their bodies at the prepupal-pupal transition. We find that betaFTZ-F1 mutants are unable to properly perform the muscle contractions that drive these processes. Several defects can be rescued by subjecting the mutants to a drop in pressure during the normal time of the prepupal-pupal transition. Our findings indicate that betaFTZ-F1 directs the muscle contraction events that drive the major morphogenetic processes during the prepupal-pupal transition in Drosophila. (C) 2003 Elsevier Science (USA). All rights reserved.