IMMUNO-CYTOCHEMICAL LOCALIZATION OF CORTICOTROPIN-RELEASING FACTOR (CRF) IN THE RAT-BRAIN
IMMUNO-CYTOCHEMICAL LOCALIZATION OF CORTICOTROPIN-RELEASING FACTOR (CRF) IN THE RAT-BRAIN
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DOI：
10.1002/aja.1001650404
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发表时间：
1982-01-01
影响因子：
--
通讯作者：
SCHALLY, AV
中科院分区：
文献类型：
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作者：
MERCHENTHALER, I;VIGH, S;SCHALLY, AV
描述了大鼠脑中含有 41 个氨基酸肽促肾上腺皮质激素释放因子 (CRF) 的神经元的免疫细胞化学定位。大鼠的秋水仙碱预处理和二氨基联苯胺终产物的银强化促进了神经元中 CRF 样免疫反应性的检测。证明了大鼠大脑所有主要分区的核周、神经元突起和末梢中存在免疫反应性 CRF。 CRF免疫反应性核周聚集体可见于下丘脑的室旁核、视上核、内侧和室周前核、乳头前核、终纹床核和前连合、内侧间隔核、伏隔核、中央杏仁核、嗅球、蓝斑、臂旁核、上核。和下丘，以及内侧前庭核。一些散在的具有CRF样免疫反应性的核周存在于室旁漏斗通路沿线、下丘脑前部、大脑皮层、海马以及中脑和脑桥的导水管周围灰质中。具有 CRF 样免疫反应性的过程存在于所有上述区域以及小脑中。 CRF 免疫反应性末端最密集的聚集出现在正中隆起的外部区域，一些免疫反应性 CRF 也存在于内部区域。含有 CRF 样免疫反应性的神经元广泛但选择性的分布支持了这种肽的神经内分泌作用，并表明 CRF 与其他神经肽类似，也可能在整个大脑中充当神经调节剂。
The immunocytochemical localization of neurons containing the 41 amino acid peptide corticotropin-releasing factor (CRF) in the rat brain is described. The detection of CRF-like immunoreactivity in neurons was facilitated by colchicine pretreatment of the rats and by silver intensification of the diaminobenzidine end-product. The presence of immunoreactive CRF in perikarya, neuronal processes and terminals in all major subdivisions of the rat brain is demonstrated. Aggregates of CRF-immunoreactive perikarya are found in the paraventricular, supraoptic, medial and periventricular preoptic, and premammillary nuclei of the hypothalamus, the bed nuclei of the stria terminalis and of the anterior commissure, the medial septal nucleus, the nucleus accumbens, the central amygdaloid nucleus, the olfactory bulb, the locus ceruleus, the parabrachial nucleus, the superior and inferior colliculus, and the medial vestibular nucleus. A few scattered perikarya with CRF-like immunoreactivity are present along the paraventriculo-infundibular pathway, in the anterior hypothalamus, the cerebral cortex, the hippocampus and the periaqueductal gray of the mesencephalon and pons. Processes with CRF-like immunoreactivity are present in all of the above areas as well as in the cerebellum. The densest accumulation of CRF-immunoreactive terminals is seen in the external zone of the median eminence, with some immunoreactive CRF also present in the internal zone. The widespread but selective distribution of neurons containing CRF-like immunoreactivity supports the neuroendocrine role of this peptide and suggests that CRF, similarly to other neuropeptides, may also function as a neuromodulator throughout the brain.