OsIPD3, an ortholog of the Medicago truncatula DMI3 interacting protein IPD3, is required for mycorrhizal symbiosis in rice
OsIPD3, an ortholog of the Medicago truncatula DMI3 interacting protein IPD3, is required for mycorrhizal symbiosis in rice
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DOI：
10.1111/j.1469-8137.2008.02612.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Zhu, Hongyan
中科院分区：
文献类型：
--
作者：
Chen, Caiyan;Ane, Jean-Michel;Zhu, Hongyan
蒺藜苜蓿IPD 3（Medicago truncatula IPD 3，MtIPD 3）是一种与DMI 3相互作用的蛋白质，DMI 3是丛枝菌根（AM）和根瘤菌共生所必需的钙/钙调素依赖性蛋白激酶（CCaMK）。然而，MtIPD 3在根共生中的功能尚未在M. truncatula，因为缺乏用于功能分析的敲除突变体。本研究利用水稻IPD 3基因敲除突变体的可利用性，对OsIPD 3基因在AM真菌共生中的功能进行了研究，筛选出3个独立的反转录转座子Tos 17插入株系，通过接种AM真菌球囊霉（Glomus intraradices）对其进行了表型和遗传分析，结果表明，OsIPD 3基因敲除突变体不能与AM真菌球囊霉（Glomus intraradices）建立共生关系。总之，IPD 3代表了植物中与AM真菌根共生所需的新基因。
Medicago truncatula IPD3 (MtIPD3) is an interacting protein of DMI3 (does not make infections 3), a Ca2+/calmodulin-dependent protein kinase (CCaMK) essential for both arbuscular mycorrhizal (AM) and rhizobial symbioses. However, the function of MtIPD3 in root symbioses has not been demonstrated in M. truncatula, because of a lack of knockout mutants for functional analysis. In this study, the availability of IPD3 knockout mutants in rice (Oryza sativa) was exploited to test the function of OsIPD3 in AM symbiosis.Three independent retrotransposon Tos17 insertion lines of OsIPD3 were selected and the phenotypes characterized upon inoculation with the AM fungus Glomus intraradices.Phenotypic and genetic analyses revealed that the Osipd3 mutants were unable to establish a symbiotic association with G. intraradices.In conclusion, IPD3 represents a novel gene required for root symbiosis with AM fungi in plants.