DNA unwinding and protein displacement by superfamily 1 and superfamily 2 helicases.
DNA unwinding and protein displacement by superfamily 1 and superfamily 2 helicases.
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DNA通过超家族1和超家族2解旋酶放松和蛋白质位移。
DOI：
10.1093/nar/gkl501
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发表时间：
2006
影响因子：
14.9
通讯作者：
Raney, Kevin D.
中科院分区：
文献类型：
--
作者：
Mackintosh, Samuel G.;Raney, Kevin D.
DNA解旋酶几乎是DNA代谢的每个方面所必需的，包括复制、修复、重组和转录。全面描述这些基本的生化过程需要详细了解解旋酶的机制。这些酶是普遍存在的，已经在病毒、原核生物和真核生物中鉴定。疾病状态，如着色性干皮病、科凯恩综合征、布卢姆综合征和沃纳综合征，已经与编码DNA解旋酶的特定基因的缺陷有关。根据序列比较，解旋酶已被放入不同的亚家族中。最大的子群被称为超家族1和超家族2。在这些类别中的解旋酶的一个建议的机制已被描述在一个“尺蠖模型”。尺蠖模型包括由ATP结合和水解驱动的构象变化，其允许沿着沿着DNA的单向移位。该酶的单体形式被认为具有两个DNA结合位点，使得DNA结合和释放的顺序步骤成为可能。解旋酶之间存在显着的差异，在重要方面的模型，如寡聚化状态的酶与一些解旋酶作为单体，一些作为二聚体和其他高阶低聚物。
DNA helicases are required for virtually every aspect of DNA metabolism, including replication, repair, recombination and transcription. A comprehensive description of these essential biochemical processes requires detailed understanding of helicase mechanisms. These enzymes are ubiquitous, having been identified in viruses, prokaryotes and eukaryotes. Disease states, such as xeroderma pigmentosum, Cockayne's syndrome, Bloom's syndrome and Werner's syndrome, have been linked to defects in specific genes coding for DNA helicases. Helicases have been placed into different subfamilies based on sequence comparison. The largest subgroups are termed superfamily 1 and superfamily 2. A proposed mechanism for helicases in these classes has been described in terms of an ‘inchworm model’. The inchworm model includes conformational changes driven by ATP binding and hydrolysis that allow unidirectional translocation along DNA. A monomeric form of the enzyme is proposed to have two DNA-binding sites that enable sequential steps of DNA binding and release. Significant differences exist between helicases in important aspects of the models such as the oligomerization state of the enzyme with some helicases functioning as monomers, some as dimers and others as higher-order oligomers.
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影响因子：
56.9
作者：
Ali, JA;Lohman, TM
通讯作者：
Lohman, TM
影响因子：
5.6
作者：
Cheng, W;Hsieh, J;Lohman, TM
通讯作者：
Lohman, TM
影响因子：
5.1
作者：
De Francesco, Raffaele;Rice, Charles M
通讯作者：
Rice, Charles M
影响因子：
16.8
作者：
Eoff, RL;Raney, KD
通讯作者：
Raney, KD
DOI：
10.1073/pnas.242479399
发表时间：
2002-12-10
影响因子：
11.1
作者：
Cheng, W;Brendza, KM;Lohman, TM
通讯作者：
Lohman, TM