MOUSE POLYCLONAL AND MONOCLONAL-ANTIBODY TO SCRAPIE-ASSOCIATED FIBRIL PROTEINS
MOUSE POLYCLONAL AND MONOCLONAL-ANTIBODY TO SCRAPIE-ASSOCIATED FIBRIL PROTEINS
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DOI：
10.1128/jvi.61.12.3688-3693.1987
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发表时间：
1987-12-01
影响因子：
5.4
通讯作者：
DIRINGER, H
中科院分区：
文献类型：
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作者：
KASCSAK, RJ;RUBENSTEIN, R;DIRINGER, H
根据抗原来源，小鼠对瘙痒症相关原纤维蛋白（蛋白酶抗性蛋白[PrP]）产生不同表位的抗体应答。小鼠对从同源（小鼠）与异源（仓鼠）物种的羊瘙痒病感染动物中分离的PrP的反应不同。建立酶联免疫吸附试验，以监测这种抗体的反应，在小鼠免疫的朊蛋白是无法检测到这样的反应，在羊瘙痒症感染的小鼠。用金黄地鼠263K PrPs免疫小鼠，获得单克隆抗体262K 3F4，该抗体与金黄地鼠PrPs发生反应，但与小鼠PrPs不发生反应。单克隆抗体263 K 3F4还识别仓鼠和人脑组织中33至35千道尔顿的正常宿主蛋白，但不识别牛、小鼠、大鼠、绵羊或兔脑。这是第一次证明不同物种中这种宿主蛋白的表位差异。通过使用单克隆抗体对PrP上的各种表位的定义将导致更好地理解PrP与其宿主前体蛋白和传染性瘙痒病因子的关系。
Antibody response in mice to scrapie-associated fibril proteins (protease-resistant proteins [PrPs]) was generated to different epitopes depending on the source of antigen. Mice responded differently to PrPs isolated from scrapie-infected animals of homologous (mouse) versus heterologous (hamster) species. An enzyme-linked immunosorbent assay established to monitor this antibody response in mice immunized with PrPs was unable to detect such a response in scrapie-infected mice. A monoclonal antibody (MAb), 262K 3F4, derived from a mouse immunized with hamster 263K PrPs reacted with hamster but not mouse PrPs. MAb 263K 3F4 also recognized normal host protein of 33 to 35 kilodaltons in brain tissue from hamsters and humans but not from bovine, mouse, rat, sheep, or rabbit brains. This is the first demonstration of epitope differences on this host protein in different species. The defining of various epitopes on PrP through the use of MAbs will lead to a better understanding of the relationship of PrPs to their host precursor protein and to the infectious scrapie agent.