Duck Tembusu virus exhibits neurovirulence in BALB/c mice.
Duck Tembusu virus exhibits neurovirulence in BALB/c mice.
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DOI：
10.1186/1743-422x-10-260
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发表时间：
2013-08-14
期刊：
影响因子：
4.8
通讯作者：
Su J
中科院分区：
文献类型：
--
作者：
Li S;Li X;Zhang L;Wang Y;Yu X;Tian K;Su W;Han B;Su J
鸭坦布苏病毒是黄病毒属Ntaya群的成员。自2010年以来，该病毒一直是中国严重鸭蛋下降综合征的罪魁祸首。它的出现和迅速传播给养禽业造成了巨大的经济损失。由于黄病毒的感染性和人畜共患病的性质，病毒感染的流行病学和对公共卫生的潜在威胁令人极为关切。本研究对鸭坦布苏病毒在BALB/c小鼠体内的致病性进行了研究。感染的小鼠在脑内接种后6天开始出现临床症状，包括食欲不振、毛发蓬乱、体重减轻、定向障碍、失明和后肢瘫痪。发病率为100%，三到八周龄小鼠的死亡率从20%到80%不等。从大脑中恢复了高滴度的病毒，病毒分布在几个器官中。组织学上，脑内有广泛的非化脓性脑炎。脾淋巴细胞耗竭，肝、肾脂肪变性。我们的结果首次证明，鸭Tembusu病毒对BALB/c小鼠具有高度的神经毒力。这项工作中使用的小鼠模型能够产生Tembusu病毒感染，并可能有助于阐明其他黄病毒感染的某些病理生理学方面。
Duck Tembusu virus is a member of the Ntaya group in the genus Flavivirus. The virus has been responsible for severe duck egg-drop syndrome in China since 2010. Its emergence and rapid spread have caused great economic loss for the poultry industry. The epidemiology of the virus infection and the potential threat to public health is of great concern because of the infective and zoonotic nature of flaviviruses. In this study, the pathogenicity of duck Tembusu virus in BALB/c mice was investigated. Infected mice developed clinical signs, including loss of appetite, ruffled hair, weight loss, disorientation, blindness and paralysis of hind limbs from six days post- infection following intracerebral inoculation. Morbidity was 100%, with mortality ranging from 20 to 80% in three- to eight-week-old mice. High virus titers were recovered from the brain, and the virus was distributed in several organs. Histologically, there was widespread non-suppurative encephalitis in the brain. Lymphocyte depletion in the spleen was observed, along with fatty degeneration in the liver and kidney. Our results demonstrate, for the first time, that duck Tembusu virus is highly neurovirulent in BALB/c mice. The mouse model used in this work was able to produce Tembusu virus infection and could be useful for elucidating some of the aspects of the pathophysiology of other flavivirus infections.