RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.
RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.
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DOI：
10.1186/s13007-016-0131-9
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发表时间：
2016
期刊：
影响因子：
5.1
通讯作者：
Salon C
中科院分区：
文献类型：
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作者：
Jeudy C;Adrian M;Baussard C;Bernard C;Bernaud E;Bourion V;Busset H;Cabrera-Bosquet L;Cointault F;Han S;Lamboeuf M;Moreau D;Pivato B;Prudent M;Trouvelot S;Truong HN;Vernoud V;Voisin AS;Wipf D;Salon C
为了在保持高产的同时节约用水和保护不可再生资源，从而限制化肥的使用，选择根系效率更高的植物是至关重要的。这可以通过优化根构型和根吸收能力和/或通过改善植物与根际微生物的积极相互作用来实现。适合于根结构高通量表型分析的设备的开发仍然是一个主要的瓶颈。描述了适合植物在受控条件下生长的根管，以及植物茎和根系统（根管）的非侵入性图像采集。这些根管可以同时生长一到六株植物，最大高度为1.1米，最长可达8周，具体取决于植物种类。在整个实验过程中，由于有一个成像舱（RhizoCab），茎部和根室都可以自动和非破坏性地成像。RhizoCab包含机器人和成像设备，用于获取植物根系的高分辨率图像。利用这个多功能的实验装置，我们说明了如何确定不同物种的一些形态计量根性状，包括模型（Medicago truncatula），作物（Pisum sativum，芸苔，葡萄，Triticum aestivum）和杂草（Vulpia myuros）物种在非限制条件下生长或受到各种非生物和生物限制。利用该系统测量的根表型性状与在盆栽中使用“经典”生长条件获得的结果进行了比较。该综合系统包括1200根根管，将允许在各种非生物和生物环境条件下进行植物芽和根的高通量表型分析。我们的系统允许一个简单的可视化或根提取和测量根性状的高通量或动力学分析。利用该系统研究根系结构将极大地促进识别作物对胁迫反应的关键根系性状的遗传和环境决定因素，包括与土壤微生物的相互作用。
In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. The development of devices suitable for high-throughput phenotyping of root structures remains a major bottleneck. Rhizotrons suitable for plant growth in controlled conditions and non-invasive image acquisition of plant shoot and root systems (RhizoTubes) are described. These RhizoTubes allow growing one to six plants simultaneously, having a maximum height of 1.1 m, up to 8 weeks, depending on plant species. Both shoot and root compartment can be imaged automatically and non-destructively throughout the experiment thanks to an imaging cabin (RhizoCab). RhizoCab contains robots and imaging equipment for obtaining high-resolution pictures of plant roots. Using this versatile experimental setup, we illustrate how some morphometric root traits can be determined for various species including model (Medicago truncatula), crops (Pisum sativum, Brassica napus, Vitis vinifera, Triticum aestivum) and weed (Vulpia myuros) species grown under non-limiting conditions or submitted to various abiotic and biotic constraints. The measurement of the root phenotypic traits using this system was compared to that obtained using “classic” growth conditions in pots. This integrated system, to include 1200 Rhizotubes, will allow high-throughput phenotyping of plant shoots and roots under various abiotic and biotic environmental conditions. Our system allows an easy visualization or extraction of roots and measurement of root traits for high-throughput or kinetic analyses. The utility of this system for studying root system architecture will greatly facilitate the identification of genetic and environmental determinants of key root traits involved in crop responses to stresses, including interactions with soil microorganisms.