Characterization of a novel β-lactamase-producing plasmid in Neisseria gonorrhoeae: sequence analysis and molecular typing of host gonococci
Characterization of a novel β-lactamase-producing plasmid in Neisseria gonorrhoeae: sequence analysis and molecular typing of host gonococci
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DOI：
10.1093/jac/dkr162
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发表时间：
2011-07-01
影响因子：
5.2
通讯作者：
Lewis, David A.
中科院分区：
文献类型：
--
作者：
Mueller, Etienne E.;Fayemiwo, Samuel A.;Lewis, David A.
目的：确定新型约翰内斯堡β-内酰胺酶编码淋球菌质粒（pEM 1）的完整核苷酸序列，并通过分子分型确定含约翰内斯堡质粒的产青霉素酶淋球菌（PPNG）的菌株相关性。先前在亚历山德拉一家诊所的男性尿道分泌物中分离的淋球菌中鉴定出11株含有约翰内斯堡b-内酰胺酶编码质粒的PPNG分离株（Johannesburg），使用PCR测定。对一个这样的质粒进行基于DNA序列的表征，以确定其与原型Asia质粒的相关性。11株含有Johannesburg质粒的PPNG分离株和105株其他临床淋球菌分离株用N.结果：质粒pEM 1由4865 bp组成，是Asia质粒的缺失衍生物，在非TnA区有2560 bp的缺失。NG-MAST基因分型表明序列类型（ST）502或其他密切相关的ST与含有约翰内斯堡质粒的PPNG之间存在显著关联（P
Objectives: To determine the complete nucleotide sequence of the novel Johannesburg beta-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing.Methods: Eleven PPNG isolates containing the Johannesburg b-lactamase-encoding plasmid were previously identified among gonococci isolated from men with urethral discharge attending a clinic in Alexandra (Johannesburg) using a PCR assay. DNA sequence-based characterization of one such plasmid was performed to determine its relatedness to the prototype Asia plasmid. The 11 PPNG isolates containing the Johannesburg plasmid and 105 other clinical gonococci isolates were typed using N. gonorrhoeae multi-antigen sequence typing (NG-MAST).Results: Plasmid pEM1 was determined to comprise 4865 bp and to be a deletion derivative of the prototype Asia plasmid with a unique 2560 bp deletion in the non-TnA region. NG-MAST genotyping demonstrated a significant association between sequence type (ST) 502, or other closely related STs, and the Johannesburg plasmid-containing PPNG (P