Immunohistochemical identification of noradrenaline‐ and adrenaline‐synthesizing neurons in the cat ventrolateral medulla
Immunohistochemical identification of noradrenaline‐ and adrenaline‐synthesizing neurons in the cat ventrolateral medulla
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猫腹外侧延髓中去甲肾上腺素和肾上腺素合成神经元的免疫组织化学鉴定
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发表时间：
1986
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影响因子：
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通讯作者：
Dong H. Park
中科院分区：
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作者：
J. Ciriello;M. M. Caverson;Dong H. Park
用荧光化学方法研究了脑池内注射秋水仙碱后猫延髓腹外侧区（VLM）含儿茶酚胺生物合成酶多巴胺β羟化酶（DBH）和苯乙醇胺N甲基转移酶（PNMT）的胞体的分布和形态。DBH免疫反应的核体被发现在整个VLM从大约脊髓交界处的水平上的上级橄榄核。在尾侧VLM中，DBH神经元主要位于外侧网状核（LRN）的背侧区域，尽管在LRN的小细胞分裂内和周围的延髓腹侧表面附近也发现了一些分散的DBH神经元。尾侧VLM中的这些DBH神经元通常为梭形、梭形双极或多极，平均体面积为422 ± 32 μ m2，具有2至4个分支突起。在VLM吻端，DBH神经元可分为三组：一组位于延髓腹外侧面面面后核腹内侧的外侧巨细胞旁核;第二组位于疑核和RFN的吻侧背内侧区域，第三组位于上级橄榄核外侧沿着。
The distribution and morphology of cell bodies containing the catecholamine biosynthetic enzymes dopamine‐β‐hydroxylase (DBH) and phenylethanolamine N‐methyltransferase (PNMT) in the ventrolateral medulla (VLM) of the cat were studied immunohistochemically after intracisternal administration of colchicine. Perikarya immunoreactive to DBH were found throughout the VLM extending from approximately the spinomedullary junction to the level of the superior olivary nucleus. In the caudal VLM DBH neurons were found primarily in the region immediately dorsal to the lateral reticular nucleus (LRN), although a few scattered DBH neurons were also found near the ventral surface of the medulla in and around the parvicellular division of the LRN. These DBH neurons in the caudal VLM were generally fusiform, fusiform‐bipolar, or multipolar, with a mean somal area of 422 ± 32 μm2, and with two to four branching processes. In the rostral VLM DBH neurons formed three distinct groups: one group was found in the nucleus paragigantocellularis lateralis in the region just ventromedial to the retrofacial nucleus (RFN) near the ventrolateral surface of the medulla; the second group was found in the region dorsomedial to the rostral aspects of the nucleus ambiguus and the RFN, and the third group was found in the region along the lateral aspect of the superior olivary nucleus.