NATURE OF INTESTINAL SLOW-WAVE FREQUENCY GRADIENT
NATURE OF INTESTINAL SLOW-WAVE FREQUENCY GRADIENT
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DOI：
10.1152/ajplegacy.1969.216.2.301
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发表时间：
1969-01-01
影响因子：
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通讯作者：
BORTOFF, A
中科院分区：
文献类型：
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作者：
DIAMANT, NE;BORTOFF, A
材料和方法这些实验大部分是在猫身上进行的，大多数基本观察在狗和恒河猴身上重复。总共使用了74只动物，包括60只猫，11只狗和3只猴子。所有动物均用戊巴比妥钠麻醉。如前所述，使用柔性吸合电极在原位和体外记录了单相慢波。在原位记录时，首先通过正中切口显露腹腔，腹壁边缘缝合到支撑金属环上。然后放置电极，并在腹腔内填充矿物油或泰罗氏溶液。常见的无感电极被放置在腹股沟或大腿的腹股沟或皮下。用辐射加热和电热垫将体温维持在37.5-38.0℃。将组织置于充气(5%COP-95%0)泰罗德溶液中进行体外实验
MATERIALS AND METHODSThe majority of these experiments were performed on cats, with most of the basic observations being repeated in dogs and rhesus monkeys. In all, 74 animals were used including 60 cats, 11 dogs, and 3 monkeys. All animals were anesthetized with sodium pentobarbital. Monophasic slow waves were recorded both in situ and in vitro with flexible suction electrodes as previously described (9). Wh en recording in situ the abdominal cavity was first exposed by a midline incision, and the edges of the abdominal wall sutured to a supporting metal ring. The electrodes were then positioned and the abdominal cavity filled with mineral oil or Tyrode’s solution. The common indifferent electrode was placed either in the abdominal cavity or subcutaneously in the groin or thigh. Body temperature was maintained at 37.5-38.0 C with radiant heat and an electric heating pad. In vitro experiments were performed with the tissue in a bath of aerated (5% COP-95% 0,) Tyrode’s solution