Eyelid Fusion and Epithelial Differentiation at the Ocular Surface During Mouse Embryonic Development
Eyelid Fusion and Epithelial Differentiation at the Ocular Surface During Mouse Embryonic Development
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DOI：
10.1007/s10384-004-0189-1
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发表时间：
2005-05
影响因子：
2.4
通讯作者：
Hui Zhang;Makiko Hara;Keisuke Seki;K. Fukuda;T. Nishida
中科院分区：
文献类型：
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作者：
Hui Zhang;Makiko Hara;Keisuke Seki;K. Fukuda;T. Nishida
目的通过检测特异性角蛋白表达的时间和空间变化来研究发育中小鼠眼表上皮细胞的分化。方法通过苏木精-伊红染色和免疫组织化学方法检测胚胎12.5-E18.5天的小鼠胚胎和成年小鼠的眼组织，包括整个眼球、结膜和眼睑，结果苏木精-伊红染色显示眼睑融合发生在E17.5。角蛋白免疫组化结果显示：（1）K4在K12之前表达，K12在K10之前表达，（2）K4、K12和K14在眼睑融合前的眼睑内陷上皮中的表达在空间上是不均匀的，而在眼睑融合后的整个结膜上皮、角膜上皮和表面上皮的基底细胞层中的表达分别是连续和均匀的;结论眼睑融合是眼表外胚层分化为结膜、角膜和眼睑皮肤上皮的关键时期。结膜上皮先于角膜上皮分化，角膜上皮又先于眼睑表皮分化。Jpn J Ophthalmol 2005;49：195-204 ©日本眼科学会2005
PurposeTo investigate epithelial differentiation at the ocular surface of the developing mouse eye by examining temporal and spatial changes in the expression of specific keratins.MethodsOcular tissues, including the entire eyeball, conjunctiva, and eyelid, of mouse embryos at embryonic day (E) 12.5 to E18.5 as well as of adult mice were examined by hematoxylin-eosin staining and by immunohistochemistry with antibodies to keratins K4, K10, K12, and K14.ResultsHematoxylin-eosin staining revealed that eyelid fusion occurred at E17.5. Keratin immunohistochemistry demonstrated that: (1) K4 was expressed before K12, which in turn was expressed before K10; (2) expression of K4, K12, and K14 was spatially heterogeneous in the epithelia of the eyelid invaginations before eyelid fusion, but thereafter was continuous and homogeneous in the entire conjunctival epithelium, corneal epithelium, and basal cell layer of the surface epithelia, respectively; and (3) K10 immunoreactivity was not detected before eyelid fusion but was apparent in the epidermis of the eyelid thereafter.ConclusionsEyelid fusion is a critical period for differentiation of the ocular surface ectoderm into the epithelia of the conjunctiva, cornea, and eyelid skin. The conjunctival epithelium differentiates before the corneal epithelium, which in turn differentiates before the eyelid epidermis.Jpn J Ophthalmol2005;49:195–204 © Japanese Ophthalmological Society 2005