THE INHERITANCE OF RESISTANCE TO METALAXYL AND TO FLUOROPHENYLALANINE IN MATINGS OF HOMOTHALLIC PHYTOPHTHORA-SOJAE
THE INHERITANCE OF RESISTANCE TO METALAXYL AND TO FLUOROPHENYLALANINE IN MATINGS OF HOMOTHALLIC PHYTOPHTHORA-SOJAE
复制标题
DOI：
10.1016/s0953-7562(09)81164-7
复制
发表时间：
1993-07-01
影响因子：
--
通讯作者：
SCHMITTHENNER, AF
中科院分区：
文献类型：
--
作者：
BHAT, RG;MCBLAIN, BA;SCHMITTHENNER, AF
对大豆疫霉耐甲酰基氟苯丙氨酸敏感(MEX（r)FPA(s)）和耐甲酰基氟苯丙氨酸敏感(MEX（s)FPA(r)）亲本及其101个F2后代进行了体外抑制剂敏感性评价。遗传证据证实，从MEX(s)FPA(r)小种1 × MEX(r)FPA(s)小种3杂交中选择的生长缓慢的单卵孢子培养物在双抑制剂萌发培养基上确实是有性杂交种。F2后代分析表明，控制梅氏菌抗性的单基因（Mex）不完全显性，而控制梅氏菌抗性的单基因（Fpa）完全显性。Mex、Fpa基因和1、3小种毒力性状独立遗传。这些数据证实了选择性表型标记在通过性周期研究Phytophtkora遗传方面的有效性。
Interracial hybrids of metalaxyl-resistant, fluorophenylalanine-sensitive (MEX(r)FPA(s)) and metalaxyl-sensitive, fluorophenylalanine-resistant (MEX(s)FPA(r)) parents of Phytopkthora sojae and 101 F2 progeny from one hybrid were evaluated in vitro for inhibitor sensitivity. Genetic evidence confirmed that a slow-growing single-oospore culture selected on a double-inhibitor germination medium from a MEX(s)FPA(r)race 1 x MEX(r)FPA(s)race 3 cross was indeed a sexual hybrid. Analysis of F2 progeny indicated that a single gene (Mex) governing MEX-resistance was incompletely dominant whereas a gene (Fpa) for FPA-resistance was fully dominant. Mex and Fpa genes and race 1 and race 3 virulence traits were inherited independently. These data confirm the usefulness of selectable phenotypic markers for studying the genetics of Phytophtkora through the sexual cycle.