Widespread occurrence of a highly conserved RING-H2 zinc finger motif in the model plant Arabidopsis thaliana
Widespread occurrence of a highly conserved RING-H2 zinc finger motif in the model plant Arabidopsis thaliana
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DOI：
10.1016/s0014-5793(98)01143-0
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发表时间：
1998-10-02
期刊：
影响因子：
3.5
通讯作者：
Skriver, K
中科院分区：
文献类型：
--
作者：
Jensen, RB;Jensen, KL;Skriver, K
经过数据库搜索后，预测了几种含有 RING-H2 锌指基序的新型拟南芥蛋白。 29 个 RING-H2 指序列的比对表明，该基序在其他不相关的蛋白质中惊人地保守。只有短的、非保守的极性/带电序列才能区分这些结构域。 RING-H2 结构域最常出现在多结构域结构中，其中许多可能包含跨膜区域或额外的锌指。然而，有几种小的（126-200 个残基）蛋白质由富含脂肪族残基的 N 端结构域和 C 端 RING-H2 结构域组成。逆转录PCR表明RING-H2基因广泛低水平表达。 (C) 1998 年欧洲生化学会联合会。
Several novel Arabidopsis thaliana proteins containing a RING-H2 zinc Finger motif were predicted after database searches. Alignment of 29 RING-H2 finger sequences shows that the motif is strikingly conserved in otherwise unrelated proteins. Only short, non-conserved polar/charged sequences distinguish these domains. The RING-H2 domain is most often present in multi-domain structures, a number of which are likely to contain a membrane-spanning region or an additional zinc finger. However, there are several small (126-200 residues) proteins consisting of an N-terminal domain, rich in aliphatic residues, and a C-terminal RING-H2 domain. Reverse-transcription PCR suggests that the RING-H2 genes are widely expressed at low levels. (C) 1998 Federation of European Biochemical Societies.