Molecular weight characterization of starch and modified starches as tricarbanilate derivatives
Molecular weight characterization of starch and modified starches as tricarbanilate derivatives
复制标题
作为三氨基苯甲酸衍生物的淀粉和改性淀粉的分子量表征
DOI：
10.1016/0144-8617(86)90003-2
复制
发表时间：
1986
影响因子：
11.2
通讯作者：
R. Klem
中科院分区：
文献类型：
--
作者：
R. Helm;R. Young;R. McPherson;R. Klem
工业玉米淀粉用氧化剂、酸或酶修饰，用高效排浆液色谱（HPSEC）技术进行了分析。该过程包括淀粉衍生成相应的三氨基甲酸酯，并通过市售聚苯二乙烯（PS-DVB）凝胶柱进行分离。分离时间在30分钟以下，在235 nm的紫外检测允许分析微克量。直链淀粉和支链淀粉三羧酸酯的洗脱行为似乎取决于允许的构象状态。支链淀粉三羧酸支链淀粉（Amylopectin tricarbanilate， APTC）被支链和洗脱层阻碍的时间远远早于线性聚苯乙烯标准柱体系的排除限。对淀粉的明显抗水解区进行了观察，并讨论了各种水解处理的效果。以单分散普鲁兰多糖的羧酸酯为主要标准，给出了相对分子量数据。
Industrial corn starches modified with an oxidant, acid or enzyme have been analyzed by a high-performance size-exclusion chromatography (HPSEC) technique. The procedure involves derivatization of the starch to the corresponding tricarbanilate with separation afforded by commercially available polystyrene-divinylbenzene (PS-DVB) gel columns. Separation time was under 30 min and UV detection at 235 nm allows the analysis of microgram quantities. The elution behavior of amylose and amylopectin tricarbanilates appears to depend on allowed conformational states. Amylopectin tricarbanilate (APTC) is hindered by branching and elutes much earlier than the exclusion limit of the column system according to linear polystyrene standards. Observation of an apparent hydrolysis resistant region of starch and the effects of the various hydrolytic treatments are discussed. Relative molecular weight data are presented utilizing the carbanilate of monodisperse pullulan polysaccharides as primary standards.