The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements
The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements
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DOI：
10.1038/nbt1239
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发表时间：
2006-09-01
影响因子：
46.9
通讯作者：
Slikker, William, Jr.
中科院分区：
文献类型：
--
作者：
Shi, Leming;Reid, Laura H.;Slikker, William, Jr.
在过去的十年中，微阵列技术的引入对基因表达研究产生了深远的影响。使用不同的微阵列平台分析相同的RNA样本，得到了不同或完全矛盾的结果，这些研究的发表引起了人们对这项技术可靠性的担忧。MicroArray质量控制（MAQC）项目的启动就是为了解决这些问题，以及其他性能和数据分析问题。使用各种基于微阵列和替代技术平台，在多个测试点生成来自两个不同参考RNA样本的四个滴定池的表达数据。在这里，我们描述了MAQC项目背后的实验设计和探针映射工作。我们展示了跨测试站点的平台内一致性以及识别为差异表达的基因方面的高水平平台间一致性。这项研究提供了一个资源，代表了在临床和监管环境中建立微阵列使用框架的重要的第一步。
Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues. Expression data on four titration pools from two distinct reference RNA samples were generated at multiple test sites using a variety of microarray-based and alternative technology platforms. Here we describe the experimental design and probe mapping efforts behind the MAQC project. We show intraplatform consistency across test sites as well as a high level of interplatform concordance in terms of genes identified as differentially expressed. This study provides a resource that represents an important first step toward establishing a framework for the use of microarrays in clinical and regulatory settings.