Corin, a mosaic transmembrane serine protease encoded by a novel cDNA from human heart
Corin, a mosaic transmembrane serine protease encoded by a novel cDNA from human heart
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DOI：
10.1074/jbc.274.21.14926
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发表时间：
1999-05-21
影响因子：
4.8
通讯作者：
Wu, QY
中科院分区：
文献类型：
--
作者：
Yan, W;Sheng, N;Wu, QY
从人类心脏中发现了一种新的cDNA，它编码一种不寻常的镶嵌丝氨酸蛋白酶，命名为corin。Corin具有II型跨膜蛋白的预测结构，并且在胞外区中含有两个卷曲样富含半胱氨酸的基序、七个低密度脂蛋白受体重复序列、一个巨噬细胞清道夫受体样结构域和一个胰蛋白酶样蛋白酶结构域。北方分析表明corin mRNA在人心脏中高表达。在小鼠中，corin mRNA通过原位杂交检测早在胚胎第9.5天的胚胎心脏的心肌细胞中。在E11.5-13.5，corin mRNA在初级房间隔和小梁心室室中最丰富。在心脏中的表达通过成人保持。此外，在发育中的骨的前肥大软骨细胞中也检测到小鼠corin mRNA。通过荧光原位杂交分析，人类corin基因被定位到4p 12 -13，其中先天性心脏病的基因座，完全异常肺静脉回流，已被定位。corin独特的结构域和胚胎期特异的表达模式提示其可能在发育过程中参与细胞分化。人类corin基因的染色体定位使其成为完全性肺静脉回流异常的一个有吸引力的候选基因。
A novel cDNA has been identified from human heart that encodes an unusual mosaic serine protease, designated corin. Corin has a predicted structure of a type II transmembrane protein and contains two frizzled-like cysteine-rich motifs, seven low density Lipoprotein receptor repeats, a macrophage scavenger receptor-like domain, and a trypsin-like protease domain in the extracellular region. Northern analysis showed that corin mRNA was highly expressed in the human heart. In mice, corin mRNA was detected by in situ hybridization in the cardiac myocytes of the embryonic heart as early as embryonic day (E) 9.5. By E11.5-13.5, corin mRNA was most abundant in the primary atrial septum and the trabecular ventricular compartment. Expression in the heart was maintained through the adult. In addition, mouse corin mRNA was also detected in the prehypertrophic chrondrocytes in developing bones. By fluorescent in situ hybridization analysis, the human corin gene was mapped to 4p12-13 where a congenital heart disease locus, total anomalous pulmonary venous return, had been previously localized. The unique domain structure and specific embryonic expression pattern suggest that corin may have a function in cell differentiation during development. The chromosomal localization of the human corin gene makes it an attractive candidate gene for total anomalous pulmonary venous return.