Histological analysis of CD11c-DTR/GFP mice after in vivo depletion of dendritic cells
Histological analysis of CD11c-DTR/GFP mice after in vivo depletion of dendritic cells
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DOI：
10.1111/j.1365-2249.2005.02868.x
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发表时间：
2005-09-01
影响因子：
4.6
通讯作者：
Van Den Broek, M
中科院分区：
文献类型：
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作者：
Probst, HC;Tschannen, K;Van Den Broek, M
为了研究个体免疫过程对DC的依赖性，已经开发了一种转基因小鼠系统（CD 11 c-DTR/GFP小鼠），其允许通过施用白喉毒素在体内有条件地消耗CD 11 c（+）DC。我们对注射白喉毒素后不同时间点的CD 11 c-DTR/ GFP小鼠进行了仔细的组织学分析，证实了淋巴结和脾脏中CD 11 c（+）细胞的短暂耗竭。出乎意料的是，白喉毒素的注射完全耗尽了脾脏的边缘区和嗜金属M Phi以及淋巴结的窦状隙对应物。这一发现限制了使用CD 11 c-DTR/ GFP小鼠分析DC对模型的作用，并证明其不依赖于边缘区和正弦M Φ。
To investigate the dependence of individual immunological processes on DC, a transgenic mouse system (CD11c-DTR/GFP mice) has been developed that allows conditional depletion of CD11c(+) DC in vivo through administration of diphtheria toxin. We have performed careful histological analysis of CD11c-DTR/ GFP mice at different time points after diphtheria toxin injection and confirmed the transient depletion of CD11c(+) cells from lymph nodes and spleen. Unexpectedly, the injection of diphtheria toxin completely depleted marginal zone and metallophilic M Phi from the spleen and their sinusoidal counterparts from the lymph nodes. This finding limits the use of CD11c-DTR/ GFP mice for the analysis of the role of DC to models and read outs that are proven to be independent of marginal zone and sinusoidal M Phi.