Leukocyte activation in atherosclerosis: Correlation with risk factors
Leukocyte activation in atherosclerosis: Correlation with risk factors
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DOI：
10.1016/s0021-9150(96)06077-7
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发表时间：
1997-05-01
期刊：
影响因子：
5.3
通讯作者：
Ohlsson, K
中科院分区：
文献类型：
--
作者：
Elneihoum, AM;Falke, P;Ohlsson, K
白细胞与动脉粥样硬化性血管疾病的发展有关，并且已经报道了许多与动脉粥样硬化有关的白细胞异常。这项针对患有早期动脉粥样硬化的中年无症状受试者的研究目的是确定白细胞活化的血浆标志物水平之间是否存在关系。即细胞因子和蛋白酶以及动脉粥样硬化的危险因素或动脉粥样硬化疾病的程度。使用 ELISA，我们测量了 156 名在超声检查中检测到无症状颈动脉斑块的受试者的中性粒细胞明胶酶相关脂质运载蛋白 (NGAL)、中性粒细胞蛋白酶 4 (NP4)（作为中性粒细胞活化标记物）、肿瘤坏死因子 α (TNF) 和可溶性 TNF 受体-1 (sTNFR-1)（作为单核细胞/巨噬细胞活化标记物）的血浆水平。血浆 TNF 和 sTNFR-1 水平与收缩压相关（分别为 r = 0.32，P < 0.04 和 r = 0.22，P < 0.05），血浆 NGAL 水平与舒张压相关（r = 0.22；P < 0.005），血浆 sTNFR-1 和 NGAL 水平与年龄（分别为 r = 0.28，P < 0.001 和 r = 0.20，P < 0.05）。与非吸烟者（n = 112）相比，吸烟者（n = 43）的血浆TNF水平（2.9 vs. 1.4 μg/l；P < 0.02）和NP4（27.5 vs. 23.4 μg/l；P < 0.05）血浆水平较高。高血压女性 (it = 7) 的血浆 NGAL 水平高于血压正常女性 (n = 85)（109 vs. 87 μg/l；P < 0.05）。因此，我们证明，在无症状的早期动脉粥样硬化受试者中，全身白细胞活化标记物的血浆水平与年龄和血压相关，并且在吸烟者和高血压患者中较高。这些结果支持了全身白细胞活化水平与动脉粥样硬化性血管疾病危险因素之间的假设关系。 (C) 1997 爱思唯尔科学爱尔兰有限公司
Leukocytes have been implicated in the development of atherosclerotic vascular diseases, and numerous abnormalities of leukocytes in conjunction with atherosclerosis have been reported. The aim of this study of middle-aged asymptomatic subjects with early atherosclerosis was to determine whether a relationship exists between the levels of plasma markers of leukocyte activation. i.e. cytokines and proteases and risk factors for atherosclerosis or the degree of atherosclerotic disease. Using ELISAs we measured the plasma levels of neutrophil gelatinase-associated lipocalin (NGAL), neutrophil protease 4 (NP4) as markers for neutrophil activation, tumor necrosis factor alpha (TNF) and soluble TNF receptor-1 (sTNFR-1) as markers of monocyte/ macrophage activation in 156 subjects with asymptomatic carotid artery plaque detected at ultrasound examination. Plasma TNF and sTNFR-1 levels were found to correlate with systolic blood pressure (r = 0.32, P < 0.04 and r = 0.22, P < 0.05, respectively), plasma NGAL level to correlate with diastolic blood pressure (r = 0.22; P < 0.005), the plasma levels of sTNFR-1 and NGAL to correlate with age (r = 0.28, P < 0.001 and r = 0.20, P < 0.05, respectively). As compared with non-smokers (n = 112), smokers (n = 43) had higher plasma levels of TNF (2.9 vs. 1.4 mu g/l; P < 0.02) and of NP4 (27.5 vs. 23.4 mu g/l; P < 0.05). The plasma NGAL level was higher in hypertensive women (it = 7) than in normotensive women (n = 85) (109 vs. 87 mu g/l; P < 0.05). We thus demonstrated that, in subjects with asymptomatic early atherosclerosis, the plasma levels of markers of systemic leukocyte activation were correlated with age and blood pressure, and were higher in smokers and hypertensives. These results support the hypothesized relationship between the level of systemic leukocyte activation and risk factors for atherosclerotic vascular disease. (C) 1997 Elsevier Science Ireland Ltd.