Degradation of indole by enrichment culture and Pseudomonas aeruginosa Gs isolated from mangrove sediment

Degradation of indole by enrichment culture and Pseudomonas aeruginosa Gs isolated from mangrove sediment
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DOI:
10.1016/j.ibiod.2005.10.001
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发表时间:
2005-12-01
影响因子:
4.8
通讯作者:
Wan, NS
Wan, NS
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
Yin, B;Gu, JD;Wan, NS

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以香港自然保护区红树林沉积物中获得的铜绿假单胞菌Gs为接种剂,研究了微生物对吲哚的降解。采用反相高效液相色谱法测定了该富集培养物和铜绿假单胞菌Gs对吲哚的降解效果。吲哚的初始浓度对降解有影响,符合零级动力学模型。铜绿假单胞菌Gs降解吲哚的最适pH为7.0,最适盐度为5‰。检测到吲哚降解的两种主要代谢物。该研究表明,吲哚可以被红树林环境中的本地微生物快速降解。(c) 2005 Elsevier Ltd版权所有。
Microbial degradation of indole was investigated using enrichment culture and Pseudomonas aeruginosa Gs obtained from mangrove sediment of Hong Kong Nature Reserve as inocula. Degradation of indole using this enrichment culture and Ps. aeruginosa Gs was quantified on reversed-phase high-performance liquid chromatography. Initial concentrations of indole affected degradation and the results conformed to the zero-order kinetic model. The optimum pH and salinity were 7.0 and 5 parts per thousand, respectively, for indole degradation by Ps. aeruginosa Gs. Two major metabolites of indole degradation were detected. This study suggests that indole can be rapidly degraded by indigenous microorganisms of the mangrove environment. (c) 2005 Elsevier Ltd. All rights reserved.