Acute and prolonged alterations in brain free magnesium following fluid percussion-induced brain trauma in rats.
Acute and prolonged alterations in brain free magnesium following fluid percussion-induced brain trauma in rats.
复制标题
大鼠液体冲击引起的脑损伤后脑游离镁的急性和长期变化。
DOI：
10.1046/j.1471-4159.1996.66062477.x
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发表时间：
1996
影响因子：
4.7
通讯作者：
McIntosh,TK
中科院分区：
文献类型：
--
作者：
Vink,R;Heath,DL;McIntosh,TK
几项研究报告称，在中枢神经系统受到创伤性侮辱后，大脑中总镁和游离镁浓度下降。尽管有证据表明，这种镁含量下降与创伤后最终的神经预后有关，但游离镁含量下降的持续时间及其对相关生物能量变量的影响相对未知。因此，本研究使用磷磁共振波谱来确定大鼠创伤性脑损伤后游离镁被抑制的时间长度。伤后即刻，脑细胞内游离镁下降至伤前的60%，并持续5天(50±8%；p<；0.001)，至伤后第8天恢复至伤前水平。伤后胞浆磷酸化率和线粒体氧化能力分别显著降低(p=0.008)和升高(p=0.002)。然而，与镁变化最大的时间不同，这些生物能量变量的最大变化出现在创伤后16-24小时，此后保持稳定，直到镁恢复。我们的结论是，创伤后游离镁的下降先于生物能量变量的变化。此外，以创伤后重建镁稳态为目标的治疗可能需要超过一周的时间。
Several studies have reported declines in brain total and free magnesium concentration after a traumatic insult to the CNS. Although the evidence suggests that this magnesium decline is associated with eventual neurologic outcome after trauma, the duration of free magnesium decline and its impact on related bioenergetic variables are relatively unknown. The present study has therefore used phosphorus magnetic resonance spectroscopy to determine the length of time that free magnesium remains suppressed after traumatic brain injury in rats. Immediately after the traumatic event, brain intracellular free magnesium declined to <60% of preinjury values and remained significantly depressed (50 ± 8%;p< 0.001) for 5 days before recovering to preinjury levels by day 8. Cytosolic phosphorylation ratio and mitochondrial oxidative capacity also significantly decreased (p= 0.008) and increased (p= 0.002), respectively, after trauma. However, unlike the time of maximum magnesium change, the maximum changes in these bioenergetic variables occurred at 16–24 h after trauma and thereafter remained stable until after the magnesium had recovered. We conclude that free magnesium decline after trauma precedes changes in bioenergetic variables. Furthermore, therapies targeted at reestablishing magnesium homeostasis after trauma may require administration over a 1‐week period.