Trace level determination of polycyclicaromatic hydrocarbons in river water withautomated pretreatment HPLC
Trace level determination of polycyclicaromatic hydrocarbons in river water withautomated pretreatment HPLC
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自动预处理高效液相色谱法测定河水中痕量多环芳烃
DOI：
10.1002/jssc.201201096
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
andK. Hosoya
中科院分区：
文献类型：
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作者：
Y. Watabe;T. Kubo;T. Tanigawa;Y. Hayakawa;K. Otsuka;andK. Hosoya
提出了一种新的多环芳烃在线预处理泵进样高效液相色谱系统。我们报告了一种新的基于泵进样HPLC的在线SPE，具有专门设计的预处理柱，用于测定地表水中痕量化学物质。众所周知，多环芳烃具有很强的致癌性，因此需要对作为自来水主要来源的饮用水和/或河水进行严格的浓度控制。我们发现可以通过使用泵进样柱切换HPLC和荧光检测来检测ng/L水平的多环芳烃。为了避免多环芳烃因其高度疏水性而经常吸附在HPLC流动线表面的现象，特别是样品输送泵中的树脂部件，我们采用了“自动稀释”装置，该装置可提供可靠的回收率和重复性。此外，收集了真实的水样，然后测定了ng/L水平的加标多环芳烃。
A novel on‐line pretreatment pump‐injection HPLC system for polycyclic aromatic hydrocarbons is proposed. We report novel pump‐injection HPLC‐based on‐line SPE with a specially designed pretreatment column for the determination of trace amounts of chemical substances in surface water. Polycyclic aromatic hydrocarbons are well known for strong carcinogenicity and thus a severe concentration control is required for drinking water and/or river water, which is the main resource of tap water. We found it possible to detect ng/L levels of polycyclic aromatic hydrocarbons by using pump‐injection column switching HPLC with fluorescence detection. To avoid the phenomenon, in which polycyclic aromatic hydrocarbons can be often adsorbed on the surface of flow lines of HPLC by their highly hydrophobicity especially resin‐made parts in sample delivery pump, we employed “autodilution” device that provides reliable recovery and repeatability. Additionally, real water samples were collected and then the spiked polycyclic aromatic hydrocarbons were determined at ng/L levels.