Extract of Lactobacillus plantarum strain 06CC2 induces JNK/p38 MAPK pathway-mediated apoptosis through endoplasmic reticulum stress in Caco2 colorectal cancer cells
Extract of Lactobacillus plantarum strain 06CC2 induces JNK/p38 MAPK pathway-mediated apoptosis through endoplasmic reticulum stress in Caco2 colorectal cancer cells
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DOI：
10.1016/j.bbrep.2019.100691
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发表时间：
2019-12-01
影响因子：
2.7
通讯作者：
Ido, Akio
中科院分区：
文献类型：
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作者：
Hiraishi, Nobuhiro;Kanmura, Shuji;Ido, Akio
结直肠癌是一种涉及遗传、环境和生活方式风险因素的多因素疾病。近年来，大肠癌患者肠道菌群的细菌组成发生了许多变化，提示肠道菌群参与了大肠癌的发生和发展。沿着这些报道，以改善肠道环境和治疗肠道疾病为目的的对人体具有有益效果的乳酸菌的研究已经取得进展。在这些研究中，biogenics（定义为来自乳酸菌的成分，无论肠道微生物菌群的状态如何，都直接作用于疾病）是最近从益生菌工作中衍生出来的概念。基于这一概念，重要的是评估来自乳酸菌的各种成分在治疗疾病中的有效性，并将其应用于预防和治疗。在这项研究中，我们研究了从植物乳杆菌菌株06 CC 2中获得的提取物对结直肠癌细胞的抗肿瘤作用。在体外实验中，与对照和非癌细胞相比，源自植物乳杆菌06 CC 2的提取物显著抑制Caco 2结肠直肠癌细胞的增殖。此外，我们发现内质网应激和JNK/p38 MAPK信号系统参与了细胞凋亡的诱导。这些发现表明植物乳杆菌06 CC 2提取物对Caco 2结直肠癌细胞的直接抗肿瘤作用，并且该提取物可能具有作为生物遗传学的潜在应用。
Colorectal cancer is a multi-factorial disease involving genetic, environmental and lifestyle risk factors. In recent years, many changes in the bacterial composition of the intestinal microflora have been reported in colorectal cancer, suggesting the involvement of the intestinal microflora in the development and progression of colorectal cancer. Along with these reports, research on lactic acid bacteria that have a beneficial effect on the human body for the purpose of improving the intestinal environment and treating intestinal diseases has advanced. Among these studies, biogenics (defined as a component derived from lactic acid bacteria that acts directly on diseases regardless of the state of intestinal microflora) is a recent concept derived from the work on probiotics. Based on this concept, it is important to evaluate the effectiveness of various components derived from lactic acid bacteria in the treatment to diseases from and apply them in prevention and treatment. In this study, we investigated the antitumor effect of an extract obtained from Lactobacillus plantarum strain 06CC2 on colorectal cancer cells. In in vitro experiments, the extract derived from Lactobacillus plantarum 06CC2 significantly suppressed the proliferation of Caco2 colorectal cancer cells in comparison to control and non-cancer cells. Furthermore, we found that endoplasmic reticulum stress and the JNK/p38 MAPK signaling system are involved in the induction of apoptosis. These findings indicate the direct antitumor effect of the Lactobacillus plantarum 06CC2 extract on Caco2 colorectal cancer cells, and that this extract may have potential application as a biogenics.