Preliminary Identification of Potential Vaccine Targets for the COVID-19 Coronavirus (SARS-CoV-2) Based on SARS-CoV Immunological Studies
Preliminary Identification of Potential Vaccine Targets for the COVID-19 Coronavirus (SARS-CoV-2) Based on SARS-CoV Immunological Studies
复制标题
DOI：
10.3390/v12030254
复制
发表时间：
2020-03-01
期刊：
影响因子：
4.7
通讯作者：
McKay, Matthew R.
中科院分区：
文献类型：
--
作者：
Ahmed, Syed Faraz;Quadeer, Ahmed A.;McKay, Matthew R.
2020年初，由新型冠状病毒严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）引起的COVID-19疫情出现。迫切需要更好地了解这种新病毒，并制定控制其传播的方法。在这项研究中，我们试图获得针对SARS-CoV-2的疫苗设计的见解，考虑SARS-CoV-2和SARS-CoV之间的高度遗传相似性，导致2003年爆发，并利用现有的SARS-CoV的免疫学研究。通过筛选SARS-CoV免疫原性结构蛋白中的实验确定的SARS-CoV衍生的B细胞和T细胞表位，我们鉴定了一组来自S蛋白和N蛋白的B细胞和T细胞表位，其与SARS-CoV-2蛋白的图谱相同。由于在120个可用的SARS-CoV-2序列中（截至2020年2月21日），在这些确定的表位中没有观察到突变，因此这些表位的免疫靶向可能会提供针对这种新型病毒的保护。对于T细胞表位，我们进行了相关MHC等位基因的人群覆盖率分析，并提出了一组表位，估计在全球以及中国提供广泛的覆盖率。我们的研究结果提供了一组筛选的表位，可以帮助指导针对SARS-CoV-2的疫苗开发的实验工作。
The beginning of 2020 has seen the emergence of COVID-19 outbreak caused by a novel coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). There is an imminent need to better understand this new virus and to develop ways to control its spread. In this study, we sought to gain insights for vaccine design against SARS-CoV-2 by considering the high genetic similarity between SARS-CoV-2 and SARS-CoV, which caused the outbreak in 2003, and leveraging existing immunological studies of SARS-CoV. By screening the experimentally-determined SARS-CoV-derived B cell and T cell epitopes in the immunogenic structural proteins of SARS-CoV, we identified a set of B cell and T cell epitopes derived from the spike (S) and nucleocapsid (N) proteins that map identically to SARS-CoV-2 proteins. As no mutation has been observed in these identified epitopes among the 120 available SARS-CoV-2 sequences (as of 21 February 2020), immune targeting of these epitopes may potentially offer protection against this novel virus. For the T cell epitopes, we performed a population coverage analysis of the associated MHC alleles and proposed a set of epitopes that is estimated to provide broad coverage globally, as well as in China. Our findings provide a screened set of epitopes that can help guide experimental efforts towards the development of vaccines against SARS-CoV-2.