The plasma membrane Na+/H+ antiporter SOS1 interacts with RCD1 and functions in oxidative stress tolerance in Arabidopsis
The plasma membrane Na+/H+ antiporter SOS1 interacts with RCD1 and functions in oxidative stress tolerance in Arabidopsis
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DOI：
10.1073/pnas.0604711103
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发表时间：
2006-12-05
影响因子：
11.1
通讯作者：
Zhu, Jian-Kang
中科院分区：
文献类型：
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作者：
Katiyar-Agarwal, Surekha;Zhu, Jianhua;Zhu, Jian-Kang
高盐胁迫对植物的不利影响包括Na+毒害、高渗胁迫和氧化胁迫。位于质膜上的Na+/H+逆向转运蛋白SOS 1在将有毒的Na+从细胞中排出的过程中起作用，并且是植物耐盐性所必需的。我们在这里报告，盐或氧化应激下，SOS 1相互作用，通过其预测的细胞质尾与RCD 1，氧化应激反应的调节器。在没有应激处理的情况下，RCD 1定位于细胞核中。在高盐或氧化胁迫下，RCD 1不仅存在于细胞核中，而且存在于细胞质中。与rcd 1突变体一样，sos 1突变体植物对氧化胁迫的敏感性也发生了改变。rcd 1突变导致sos 1突变体植物耐盐性下降，并增强了盐胁迫敏感性。与氧化应激耐受性相关的几个基因被发现由RCD 1和SOS 1调节。这些结果揭示了一个以前未知的功能质膜Na+/H+逆向转运蛋白在氧化胁迫耐受性和阐明离子稳态和氧化胁迫解毒途径之间的串扰参与植物耐盐性。
The adverse effects of high salt on plants include Na+ toxicity and hyperosmotic and oxidative stresses. The plasma membrane-localized Na+/H+ antiporter SOS1 functions in the extrusion of toxic Na+ from cells and is essential for plant salt tolerance. We report here that, under salt or oxidative stress, SOS1 interacts through its predicted cytoplasmic tail with RCD1, a regulator of oxidative-stress responses. Without stress treatment, RCD1 is localized in the nucleus. Under high salt or oxidative stress, RCD1 is found not only in the nucleus but also in the cytoplasm. Like rcd1 mutants, sos1 mutant plants show an altered sensitivity to oxidative stresses. The rcd1mutation causes a decrease in salt tolerance and enhances the salt-stress sensitivity of sos1 mutant plants. Several genes related to oxidative-stress tolerance were found to be regulated by both RCD1 and SOS1. These results reveal a previously uncharacterized function of a plasma membrane Na+/H+ antiporter in oxidative-stress tolerance and shed light on the cross-talk between the ion-homeostasis and oxidative-stress detoxification pathways involved in plant salt tolerance.