Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38
Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38
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DOI：
10.1007/s10811-023-03136-3
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发表时间：
2023-12-07
影响因子：
3.3
通讯作者：
Adams，Jessica M. M.
中科院分区：
文献类型：
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作者：
Rodrigues，Valerie J.;Jouanneau，Diane;Adams，Jessica M. M.
石莼多糖是一种绿色大型藻类细胞壁多糖，由于其硫酸化鼠李糖、葡萄糖醛酸、艾杜糖醛酸和木糖的独特组成，具有巨大的价值增值潜力。几种潜在的应用，如生产生物燃料，生物塑料和其他增值产品，需要将多糖分解为低聚物或单体。在过去的十年中，对石莼多糖糖化酶的研究一直在不断增加，越来越多地关注海藻生物质的生物基经济价值。裂合酶是参与多糖糖化的几种酶中的第一种，并且几种石莼多糖裂合酶已经在结构上和生物化学上表征，以使它们能够有效地用于稳定过程。本研究对石莼多糖代谢菌Vibriosp.FNV38的全基因组进行了研究，并对其所含的石莼多糖裂解酶PL 24的生化特性进行了分析。Vibriosp.FNV38的基因组具有多样的CAZy谱，其中几个基因参与石莼多糖、纤维素、琼脂和藻酸盐的代谢。该酶在100 mM Tris-HCl缓冲液中pH 8.5和30 °C下表现出最佳活性。然而，其热稳定性差，在高于25 °C的温度下孵育2小时后活性显著丧失。分解产物分析表明，酶解聚的多糖主要为二糖和四糖。
Ulvan is a green macroalgal cell wall polysaccharide that has tremendous potential for valorisation due to its unique composition of sulphated rhamnose, glucuronic acid, iduronic acid and xylose. Several potential applications such as production of biofuels, bioplastics and other value-added products necessitate the breakdown of the polysaccharide to oligomers or monomers. Research on ulvan saccharifying enzymes has been continually increasing over the last decade, with the increasing focus on valorisation of seaweed biomass for a biobased economy. Lyases are the first of several enzymes that are involved in saccharifying the polysaccharide and several ulvan lyases have been structurally and biochemically characterised to enable their effective use in the valorisation processes. This study investigates the whole genome ofVibriosp.FNV38, an ulvan metabolising organism and biochemical characteristics of a PL24 ulvan lyase that it possesses. The genome ofVibriosp.FNV38 has a diverse CAZy profile with several genes involved in the metabolism of ulvan, cellulose, agar, and alginate. The enzyme exhibits optimal activity at pH 8.5 in 100 mM Tris–HCl buffer and 30 °C. However, its thermal stability is poor with significant loss of activity after 2 h of incubation at temperatures above 25 °C. Breakdown product analysis reveals that the enzyme depolymerised the polysaccharide predominantly to disaccharides and tetrasaccharides.