Reproducibility and clinical application of a newly developed stabilized retinal laser Doppler instrument
Reproducibility and clinical application of a newly developed stabilized retinal laser Doppler instrument
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DOI：
10.1016/s0002-9394(02)01949-9
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发表时间：
2003-03-01
影响因子：
4.2
通讯作者：
McMeel, JW
中科院分区：
文献类型：
--
作者：
Yoshida, A;Feke, GT;McMeel, JW
目的：描述一种新开发的稳定视网膜激光多普勒仪器，报告视网膜血流测量的可重复性，并介绍其临床应用实例。设计：实验研究。方法：使用佳能激光血流仪CLBF 100模型评估视网膜血流测量的内、间和观察者间的可重复性。在6名健康志愿者的双眼中，计算了视网膜血管直径(D)、时间平均中心线血流速度（V-av）和血流(F)的重复测量（5X）的变异系数（CV），分别位于视网膜颞动脉和视网膜颞静脉的18个位置。间歇：计算同一名检查人员在两个不同的日子对同一36个视网膜位置进行的两组测量的相关系数和平均差值。观察者间：计算两名检查人员在同一天对5名志愿者的5只眼睛的9个部位（5个动脉和4个静脉）进行的两组测量的相关系数和平均差值。视网膜分支静脉阻塞（BRVO）和视网膜中央静脉阻塞（CRVO）两例患者的结果表明视网膜循环特征异常。结果:Intrasession:简历(意思是+ / - SD) D, V-av,和F + / - 2.2%, 5.5% 13.1% + / - 6.3%,和13.8% + / - 4.7%,动脉和3.5% + / - 2.1%,11.9% + / - 5.9%,和12.7%,静脉+ / - 5.5%。间歇/观察者间：在D、Vav和F中，一名审查员在两个不同的日期和两名审查员在同一天的测量结果之间存在很强的相关性。在间歇比较中，F的平均差异为16.4% +/- 12.8%，在观察者间比较中，F的平均差异为12.6% +/- 7.0%。BRVO患者的结果显示，在同一只眼睛中，与未受影响的象限相比，受影响象限的血流特征有显著差异，与另一只眼睛相比也是如此。CRVO患者的结果显示清除闭塞后血流特性显著改善。结论：再现性结果和以实际单位（多/分钟）测量血流的事实表明，该仪器可用于可靠地比较同眼不同视网膜血管部位、双眼可比部位的血流特征，并可用于患者与健康对照者之间的比较。(C) 2003年由爱思唯尔科学有限公司版权所有。
PURPOSE: To describe a newly developed stabilized retinal laser Doppler instrument, to report the reproducibility of retinal blood flow measurements, and to present examples of its clinical application.DESIGN: Experimental study.METHODS: The intrasession, intersession, and interobserver reproducibility of retinal blood flow measurements obtained using the Canon Laser Blood Flowmeter model CLBF 100 was assessed. Intrasession: the coefficients of variation (CV) for repeated measurements (5X) of retinal vessel diameter (D), time-average centerline blood velocity (V-av), and blood flow (F) were calculated at 18 sites along temporal retinal arteries and 18 sites along temporal retinal veins using both eyes in six healthy volunteers. Intersession: the correlation coefficients and average differences between two sets of measurements at the same 36 retinal sites made by one examiner on two different days were calculated. Interobserver: the correlation coefficients and average differences between two sets of measurements at nine sites (five arterial and four venous) in five eyes of five of the volunteers made by two examiners on the same day were calculated. Results from two patients, one with branch retinal vein occlusion (BRVO), and one with central retinal vein occlusion (CRVO), are used to illustrate abnormal retinal circulatory characteristics.RESULTS: Intrasession: CV (mean +/- SD) for D, V-av, and F were 5.5% +/- 2.2%,13.1% +/- 6.3%, and 13.8% +/- 4.7% in arteries and 3.5% +/- 2.1%, 11.9% +/- 5.9%, and 12.7%, +/- 5.5% in veins. Intersession/interobserver: strong correlations between measurements made by one examiner on two different days and by two examiners on the same day were found in D, Vav, and F. Average differences in F were 16.4% +/- 12.8% for intersession and 12.6% +/- 7.0% for interobserver comparisons. Results from the patient with BRVO show marked differences in flow characteristics in the affected quadrant compared with an unaffected quadrant in the same eye, and to the fellow eye. Results from the patient with CRVO show a dramatic improvement in flow characteristics after clearing of the occlusion.CONCLUSIONS: The reproducibility results and the fact that blood flow is measured in actual units of mul/min indicate that the instrument can be used for reliable comparison of blood flow characterictics at different retinal vascular sites in the same eye, at comparable sites in both eyes, and for comparison between patients and healthy control subjects. (C) 2003 by Elsevier Science Inc. All rights reserved.