Highly conducting gold nanoparticles-graphene nanohybrid films for ultrasensitive detection of carcinoembryonic antigen.
Highly conducting gold nanoparticles-graphene nanohybrid films for ultrasensitive detection of carcinoembryonic antigen.
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DOI：
10.1016/j.talanta.2011.03.036
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发表时间：
2011-07
期刊：
影响因子：
6.1
通讯作者：
Jing Han;Ying Zhuo;Y. Chai;Li Mao;Ya-Li Yuan;R. Yuan
中科院分区：
文献类型：
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作者：
Jing Han;Ying Zhuo;Y. Chai;Li Mao;Ya-Li Yuan;R. Yuan
基于壳聚糖-二茂铁（CS-Fc）和纳米TiO 2（CS-Fc + TiO 2）复合膜及金纳米颗粒-石墨烯（Au-Gra）纳米杂化材料，构建了一种检测癌胚抗原（CEA）的新型免标记安培免疫传感器。首次将CS-Fc + TiO 2复合膜修饰在裸玻碳电极上。然后通过自组装策略在CS-Fc + TiO 2膜上形成Au-Gra纳米杂化物。其次，根据Au-Gra与抗CEA抗体的氨基之间的强相互作用，构建了抗CEA抗体的进一步固定化。由于Au-Gra纳米杂化膜为生物分子的固定提供了一个适宜的微环境，因此可以提高抗体蛋白的表面覆盖度，提高免疫传感器的灵敏度。石墨烯纳米片和Au NPs的协同效应可能是其良好导电特性的原因。采用扫描电镜（SEM）和循环伏安法（CV）对改性过程进行了表征。在优化的条件下，所得到的生物传感器显示良好的安培响应CEA的线性范围为0.01至80 ng/mL和3.4 pg/mL的检测限（信号/噪声= 3）。结果表明，该免疫传感器具有导电性好、灵敏度高、寿命长等优点。该方法在临床应用和低水平蛋白质检测方面显示了巨大的潜力。
A new label-free amperometric immunosensor was developed for detection of carcinoembryonic antigen (CEA) based on chitosan-ferrocene (CS-Fc) and nano-TiO2(CS-Fc + TiO2) complex film and gold nanoparticles–graphene (Au–Gra) nanohybrid. CS-Fc + TiO2composite membrane was first modified on a bare glass carbon electrode. Then Au–Gra nanohybrid was formed on the CS-Fc + TiO2membrane by self-assembly strategy. Next, further immobilization of anti-CEA was constructed according to the strong interaction between Au–Gra and the amido groups of anti-CEA. Since Au–Gra nanohybrid films provided a congenial microenvironment for the immobilization of biomolecules, the surface coverage of antibody protein could be enhanced and the sensitivity of the immunosensor has been improved. The good electronic conductive characteristic might be attributed to the synergistic effect of graphene nanosheets and Au NPs. The modified process was characterized by scanning electron microscope (SEM) and cyclic voltammetry (CV). Under optimized conditions, the resulting biosensor displayed good amperometric response to CEA with linear range from 0.01 to 80 ng/mL and a detection limit of 3.4 pg/mL (signal/noise = 3). The results demonstrated that the immunosensor has advantages of high conduction, sensitivity, and long life time. This assay approach showed a great potential in clinical applications and detection of low level proteins.