NONMYELIN-FORMING SCHWANN-CELLS COEXPRESS SURFACE-PROTEINS AND INTERMEDIATE FILAMENTS NOT FOUND IN MYELIN-FORMING CELLS - A STUDY OF RAN-2, A5E3 ANTIGEN AND GLIAL FIBRILLARY ACIDIC PROTEIN
NONMYELIN-FORMING SCHWANN-CELLS COEXPRESS SURFACE-PROTEINS AND INTERMEDIATE FILAMENTS NOT FOUND IN MYELIN-FORMING CELLS - A STUDY OF RAN-2, A5E3 ANTIGEN AND GLIAL FIBRILLARY ACIDIC PROTEIN
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DOI：
10.1007/bf01148594
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
MIRSKY, R
中科院分区：
文献类型：
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作者：
JESSEN, KR;MIRSKY, R
人们对周围神经系统中非髓鞘形成神经胶质的分子特性知之甚少。这些细胞和中枢神经系统神经胶质细胞之间的关系知之甚少，与髓鞘形成的许旺细胞和少突胶质细胞之间的生物化学和功能相似性形成鲜明对比。星形胶质细胞上发现的两种细胞表面蛋白，Ran-2和A5 E3抗原，以及中间丝蛋白，胶质细胞酸性蛋白（GFAP），都广泛表达于成年大鼠各种神经中的非髓鞘形成的雪旺细胞原位，但不是髓鞘形成的雪旺细胞。研究了这3种蛋白质是否仅由成年神经中原位的相同雪旺细胞群共表达，或者是否可以定义非髓鞘形成雪旺细胞亚群表达1种或2种但不是所有3种抗原。2个表面蛋白，RAN-2和A5 E3抗原，均由单克隆抗体定义的分布进行比较，在周围神经干的成年大鼠使用双标记免疫荧光细胞内中间丝蛋白GFAP的分布。所有3种蛋白质的表达仅限于相同的许旺细胞群，即无髓鞘轴突周围的许旺细胞。围绕较大轴突的形成髓鞘的许旺细胞不表达化学检测量的任何蛋白质。在戏弄的神经准备从节前交感神经干，坐骨神经，臂丛，背根和腹根，一个完整的对应关系被发现之间的表达Ran-2和GFAP，和A5 E3和GFAP之间的非髓鞘形成的雪旺细胞。未发现单独的Ran-2、A5 E3抗原或GFAP阳性的雪旺氏细胞。同样的抗原群也在成年大鼠CNS中的星形胶质细胞和PNS中的肠胶质细胞中表达，而在其他类型的细胞中不表达。因此，表型A5 E3+、Ran-2+、GFAP+是成年大鼠PNS和CNS神经胶质细胞的主要类别的特征，表明这可能反映了这3种细胞类型的一些共同功能。
Little is known about the molecular properties of the nonmyelin-forming glia of the PNS [peripheral nervous system]. The relationship between these cells and CNS glia is poorly understood, in contrast with the well-documented biochemical and functional similarities between myelin-forming Schwann cells and oligodendrocytes. Two cell surface proteins found on astrocytes, Ran-2 and A5E3 antigen, and the intermediate filament protein, glial fibrillary acidic protein (GFAP), are all widely expressed in situ by nonmyelin-forming Schwann cells in various nerves from adult rats, but not by myelin-forming Schwann cells. Whether these 3 proteins are exclusively coexpressed by the same population of Schwann cells in situ in adult nerves, or alternatively whether subpopulations of nonmyelin-forming Schwann cells can be defined that express 1 or 2 but not all 3 of these antigens was investigated. The distribution of 2 surface proteins, Ran-2 and A5E3 antigen, both defined by monoclonal antibodies, is compared with the distribution of the intracellular intermediate filament protein GFAP in peripheral nerve trunks of adult rats using double label immunofluorescence. The expression of all 3 proteins is confined to the same population of Schwann cells, namely, the Schwann cells surrounding the unmyelinated axons. The myelin-forming Schwann cells surrounding the larger axons do not express immunohistochemically detectable amounts of any of the proteins. In teased nerve preparations from the preganglionic sympathetic trunk, sciatic nerve, brachial plexus, dorsal and ventral roots, a complete correspondence is found between expression of Ran-2 and GFAP, and between A5E3 and GFAP in nonmyelin-forming Schwann cells. No Schwann cells positive for Ran-2 alone, A5E3 antigen alone, or GFAP alone were found. The same constellation of antigens is also expressed by astrocytes in the adult rat CNS and enteric glia in the PNS and not by other cell types. The phenotype A5E3+, Ran-2+, GFAP+ is thus characteristic of major categories of adult rat PNS, and CNS glial cells, suggesting that this may reflect some common function for these 3 cell types.