Adult neurogenesis occurs in primate sensorimotor cortex following cervical dorsal rhizotomy.
Adult neurogenesis occurs in primate sensorimotor cortex following cervical dorsal rhizotomy.
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DOI：
10.1523/jneurosci.5272-09.2010
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发表时间：
2010-06-23
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影响因子：
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通讯作者：
Darian-Smith C
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文献类型：
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作者：
Vessal M;Darian-Smith C
在大脑皮层中，成人神经发生仍然存在争议。我们以前在猴子和大鼠身上发现，颈髓背根切断术后6-8周，脊髓背角会发生反应性神经再生。在这里，在三只有相同病变的猴子身上，我们询问它是否也巧合地发生在相应的初级躯体感觉和运动皮质中，在那里已知发生了重大的地形和神经元重组。雄性短尾猴断根后2~3周注射溴脱氧尿嘧啶核苷(BrdU)，4~6周后灌流。在初级躯体感觉和运动皮质内观察到BrdU标记的细胞和5种不同的神经元标志物，并对它们的分布进行了双侧比较。用BrdU和星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)标记的细胞也进行了定量比较。在局部重组的皮质中观察到大量BrdU/Neun和BrdU/calbindin(CB)标记细胞。双侧也可观察到少量BrdU/GFAP突起细胞，但这些细胞从未与任何神经元标记物并列。在用BrdU和神经元标记物并列标记的细胞中，至少有一半具有抑制表型。然而，兴奋性锥体神经元也具有典型的锥体形态。在其他皮质区域未观察到皮质神经发生。在另一只没有手术干预的对照猴(雄性猕猴)的初级感觉运动、前额叶或后顶叶皮质中也没有观察到这种现象。我们的发现为背根切断后的反应性内源性皮质神经发生提供了证据，这可能在功能恢复中发挥作用。
Adult neurogenesis remains controversial in the cerebral cortex. We have previously shown in monkeys and rats that reactive neurogenesis occurs in the spinal dorsal horn 6–8 weeks following a cervical dorsal rhizotomy. Here, in three monkeys with the same lesion, we asked whether it also occurs coincidentally in the corresponding primary somatosensory and motor cortex, where significant topographic and neuronal reorganization is known to occur. Monkeys (male Macaca fascicularis) were given bromodeoxyuridine (BrdU) injections 2–3 weeks post-rhizotomy, and perfused 4–6 weeks later. Cells colabeled for BrdU and five different neuronal markers were observed within the primary somatosensory and motor cortex and their distributions, were compared bilaterally. Cells colabeled with BrdU and the astrocytic marker glial fibrillary acidic protein (GFAP) were also quantified for comparison. A significant number of BrdU/NeuN and BrdU/calbindin (CB) colabeled cells were observed in topographically reorganized cortex. Small numbers of BrdU/GFAP colabed cells were also consistently observed bilaterally, but these cells never colabeled with any of the neuronal markers. Of the cells colabeled with BrdU and a neuronal marker, at least half had an inhibitory phenotype. However, excitatory pyramidal neurons were also identified with classic pyramidal morphology. Cortical neurogenesis was not observed in other cortical regions. It was also not observed in the primary sensorimotor, prefrontal, or posterior parietal cortex in an additional control monkey (male Macaca fascicularis) that had no surgical intervention. Our findings provide evidence for reactive endogenous cortical neurogenesis following a dorsal rhizotomy, which may play a role in functional recovery.