Effects of co-overproduction of Rubisco and chloroplast glyceraldehyde-3-phosphate dehydrogenase on photosynthesis in rice
Effects of co-overproduction of Rubisco and chloroplast glyceraldehyde-3-phosphate dehydrogenase on photosynthesis in rice
复制标题
Rubisco与叶绿体甘油醛-3-磷酸脱氢酶共同过量产生对水稻光合作用的影响
DOI：
10.1080/00380768.2021.1915100
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发表时间：
2021
影响因子：
2
通讯作者：
Miyake C and Makino A
中科院分区：
文献类型：
--
作者：
Suzuki Y*;Ishiyama K;Cho A;Takegahara-Tamakawa Y;Wada S;Miyake C and Makino A
Rubisco 底物核酮糖 1,5-二磷酸 (RuBP) 的再生能力是 Rubisco 过量生产的转基因水稻植物光合作用瓶颈的候选者之一。由于叶绿体3-磷酸甘油醛脱氢酶（GAPDH）的过量生产被认为会稍微增强RuBP再生能力，因此产生了同时过量生产Rubisco和GAPDH的转基因水稻植物以改善光合作用。 Rubisco 的量增加了 8% 至 18%，而 GAPDH 活性则增加至野生型的 2.1 至 3.2 倍。然而，在高辐照度和不同[CO2]分压的条件下，CO2同化速率并没有增加。讨论了共同过量生产植物中光合作用受到 Rubisco、GAPDH 和其他未知因素共同限制的可能性。
The capacity for regeneration of the substrate for Rubisco, ribulose 1,5-bisphosphate (RuBP), is one of the candidates for the bottleneck in photosynthesis in transgenic rice plants with overproduction of Rubisco. As the overproduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was suggested to slightly enhance RuBP regeneration capacity, transgenic rice plants with co-overproduction of Rubisco and GAPDH were generated to improve photosynthesis. The amount of Rubisco increased by 8% to 18%, whereas GAPDH activity increased to 2.1- to 3.2-fold that of the wild-type. However, the rate of CO2assimilation did not increase under conditions of high irradiance and different [CO2] partial pressures. The possibility that photosynthesis in the co-overproduced plants was co-limited by Rubisco, GAPDH, and other unknown factors is discussed.