In vitro comparison of immunological properties of cultured human mesenchymal cells from various sources
In vitro comparison of immunological properties of cultured human mesenchymal cells from various sources
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DOI：
10.1007/s10517-008-0057-y
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发表时间：
2008-02-01
影响因子：
0.7
通讯作者：
Yarygin, K. N.
中科院分区：
文献类型：
--
作者：
Suzdaltseva, Yu. G.;Burunova, V. V.;Yarygin, K. N.
体外研究了骨髓间充质干细胞、新生儿脐带成纤维细胞样细胞、成人供者皮肤成纤维细胞与外周血单核细胞相互作用过程中细胞介导的细胞毒作用。与来源无关，间充质细胞不与同种异体自然杀伤细胞和细胞毒性淋巴细胞裂解。间充质细胞混合培养对外周血单核细胞无细胞毒作用，对T、B淋巴细胞、自然杀伤细胞和CD14(+)淋巴细胞的增殖无激活作用。体外实验表明，不同来源的间充质细胞与同种异体免疫活性细胞具有较强的耐受性。
Cell-mediated cytotoxicity was studied in vitro during the interaction of bone marrow mesenchymal stem cells, fibroblast-like cells from newborn umbilical cord, and skin fibroblasts of an adult donor with peripheral blood mononuclear cells. Independently on the origin, mesenchymal cells were not lysed with allogeneic natural killer cells and cytotoxic lymphocytes. Mixed cultures of mesenchymal cells had no cytotoxic effect on peripheral blood mononuclear cells and did not activate proliferation of T and B lymphocytes, natural killer cells, and CD14(+) lymphocytes. In vitro experiments showed that mesenchymal cells of different origin and allogeneic immunocompetent cells are tolerant to each other.