G-CSF-induced macrophage polarization and mobilization may prevent acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
G-CSF-induced macrophage polarization and mobilization may prevent acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
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G-CSF诱导的巨噬细胞极化和动员可以预防异基因造血干细胞移植后的急性移植物抗宿主病
DOI：
10.1038/s41409-019-0449-9
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发表时间：
2019-09-01
影响因子：
4.8
通讯作者：
Huang, Xiao-Jun
中科院分区：
文献类型：
--
作者：
Wen, Qi;Kong, Yuan;Huang, Xiao-Jun
巨噬细胞（M Phi s）是一种重要的免疫细胞群，对组织稳态和疾病发病机制至关重要。M Phi现在被分类为产生促炎细胞因子的M1或产生促炎细胞因子的M2。粒细胞集落刺激因子（G-CSF）对人类M Phi s的影响尚不清楚。此外，很少有人知道之间的关联M φ亚群在同种异体移植物和急性移植物抗宿主病（aGVHD）的患者进行异基因造血干细胞移植（allo-HSCT）的发生。在目前的研究中，我们发现，M1/M2比值显着降低，在G-CSF治疗的骨髓（后BM）和G-CSF治疗的外周血从健康供体。BM后M Phi表现出迁移减少和吞噬作用增加。此外，BM后M Phi s降低了Th 1和Tc 1谱系的百分比，并增加了Th 2、Tc 2和Treg谱系的百分比。接受具有较高M1/M2比率的BM移植物的患者表现出较高的2-4级aGVHD发生率。总之，我们的数据表明，G-CSF降低了来自健康供体的BM移植物中的M1/M2比率，这可能有助于防止allo-HSCT后患者中2-4级aGVHD的发生。
Macrophages (M Phi s) are an important immune cell population that are essential for tissue homeostasis and disease pathogenesis. M Phi s are now classified as either M1, which produce pro-inflammatory cytokines, or M2, which produce antiinflammatory cytokines. The impact of granulocyte colony-stimulating factor (G-CSF) on M Phi s in humans is unclear. Moreover, little is known about the association between M Phi subsets in allografts and the occurrence of acute graft-versushost disease (aGVHD) in patients who undergo allogeneic hematopoietic stem cell transplantation (allo-HSCT). In the current study, we found that the M1/M2 ratio was markedly decreased in both G-CSF-treated bone marrow (post-BM) and G-CSF-treated peripheral blood from healthy donors. Post-BM M Phi s exhibited reduced migration and increased phagocytosis. Moreover, post-BM M Phi s reduced the percentage of Th1 and Tc1 lineages and increased the percentage of Th2, Tc2, and Treg lineages. Patients who received BM grafts with a higher M1/M2 ratio exhibited a higher incidence of grade 2-4 aGVHD. In summary, our data indicate that G-CSF decreases the M1/M2 ratio in BM grafts from healthy donors, which may contribute to preventing the occurrence of grade 2-4 aGVHD in patients after allo-HSCT.