Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds
Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds
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DOI：
10.1016/j.biomaterials.2004.10.005
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发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Jayakrishnan, A
中科院分区：
文献类型：
--
作者：
Balakrishnan, B;Jayakrishnan, A
可注射性高分子支架具有良好的生物相容性和生物降解性，是组织工程和药物释放的重要材料。来源于天然蛋白质和多糖的水凝胶是组织工程的理想支架，因为它们类似于由各种氨基酸和糖基大分子组成的组织的细胞外基质。在这里，我们报告了一类新的水凝胶衍生自氧化海藻酸盐和明胶。我们表明，高碘酸盐氧化的海藻酸钠具有适当的分子量和氧化程度迅速交联蛋白质，如明胶在低浓度的四硼酸钠（Na 2B）的存在下，给组织工程，药物输送和其他医疗应用的可注射系统。在β-淀粉酶存在下的快速凝胶化归因于介质的微碱性pH以及β-淀粉酶与多糖的羟基络合的能力。考察了海藻酸二醛、明胶和海藻酸钠的氧化程度、浓度对凝胶化速度的影响。作为一般规则，胶凝时间随着氧化的海藻酸盐、明胶和明胶的浓度的增加以及海藻酸盐的氧化程度的增加而减少。凝胶基质的交联参数进行了研究，通过溶胀测量和三硝基苯磺酸（TNBS）测定。在一般情况下，交联度被认为是增加与海藻酸盐的氧化程度的增加，而溶胀比和溶胀度下降。发现该凝胶具有生物相容性和生物可降解性。通过使用伯氨喹作为模型药物和通过将肝细胞封装在凝胶基质内，分别证明了该系统作为可注射药物递送载体和作为组织工程支架的潜力。(C)2004 Elsevier Ltd.保留所有权利。
The injectable polymer scaffolds which are biocompatible and biodegradable are important biomaterials for tissue engineering and drug delivery. Hydrogels derived from natural proteins and polysaccharides are ideal scaffolds for tissue engineering since they resemble the extracellular matrices of the tissue comprised of various amino acids and sugar-based macromolecules. Here, we report a new class of hydrogels derived from oxidized alginate and gelatin. We show that periodate-oxidized sodium alginate having appropriate molecular weight and degree of oxidation rapidly cross-links proteins such as gelatin in the presence of small concentrations of sodium tetraborate (borax) to give injectable systems for tissue engineering, drug delivery and other medical applications. The rapid gelation in the presence of borax is attributed to the slightly alkaline pH of the medium as well as the ability of borax to complex with hydroxyl groups of polysaccharides. The effect of degree of oxidation and concentration of alginate dialdehyde, gelatin and borax on the speed of gelation was examined. As a general rule, the gelling time decreased with increase in concentration of oxidized alginate, gelatin and borax and increase in the degree of oxidation of alginate. Cross-linking parameters of the gel matrix were studied by swelling measurements and trinitrobenzene sulphonic acid (TNBS) assay. In general, the degree of cross-linking was found to increase with increase in the degree of oxidation of alginate, whereas the swelling ratio and the degree of swelling decreased. The gel was found to be biocompatible and biodegradable. The potential of the system as an injectable drug delivery vehicle and as a tissue-engineering scaffold is demonstrated by using primaquine as a model drug and by encapsulation of hepatocytes inside the gel matrix, respectively. (C) 2004 Elsevier Ltd. All rights reserved.