Surfactant protein D is a useful biomarker for monitoring acute lung injury in rats
Surfactant protein D is a useful biomarker for monitoring acute lung injury in rats
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DOI：
10.1080/01902148.2016.1215570
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发表时间：
2016-01-01
影响因子：
1.7
通讯作者：
Takahashi, Hiroki
中科院分区：
文献类型：
--
作者：
Murata, Makoto;Otsuka, Mitsuo;Takahashi, Hiroki
目的：本研究旨在观察博莱霉素诱导的肺损伤大鼠模型血清和支气管肺泡灌洗液（BALF）中SP-D浓度的动态变化，并与常规生化指标进行比较。材料与方法：雄性Wister大鼠麻醉后，气管内注射博莱霉素（1.0mg/kg）。观察肺组织病理学改变及SP-D表达。我们还测量了SP-D，白蛋白和乳酸脱氢酶（LDH）的浓度和BALF中各种类型细胞的数量，以及SP-D和常规标志物（包括LDH，高迁移率族蛋白1，单核细胞趋化蛋白1和C反应蛋白）的血清水平。结果：BALF中SP-D水平升高，第3天达高峰，随后逐渐下降。这些变化与BALF白蛋白水平和粒细胞计数的变化显著相关。血清SP-D水平从第5天开始升高，在第10天达到峰值，然后逐渐下降，直到第28天。血清SP-D水平的变化准确反映了肺损伤引起的组织学改变的程度。另一方面，常规生物标志物的血清水平仅在几天内升高或在研究期间没有变化。结论：SP-D水平是反映肺损伤严重程度的重要指标。这些结果表明，SP-D水平的测量是一个额外的工具，监测大鼠急性肺损伤。
Purpose: The purpose of this study is to investigate the serial changes in the SP-D concentrations of serum and bronchoalveolar lavage fluid (BALF) in a bleomycin-induced lung injury rat model and compare them with the levels of conventional biochemical markers. Materials andMethods: MaleWister rats were anesthetized and intratracheally administered bleomycin (1.0 mg/kg). We evaluated the histological changes and SP-D expression of their lung tissues. We also measured the concentrations of SP-D, albumin, and lactate dehydrogenase (LDH) and the numbers of various types of cells in BALF, and the serum levels of SP-D and conventional markers, including LDH, high mobility group box 1, monocyte chemoattractant protein-1, and C-reactive protein. Results: The BALF SP-D level increased and peaked on day 3, and then gradually decreased. These variations were significantly correlated with the changes in the BALF albumin level and granulocyte cell count. The serum SP-D level increased from day 5, peaked on day 10, and then gradually decreased until day 28. The changes in the serum SP-D level accurately reflected the extent of the histological changes caused by the lung injury. On the other hand, the serum levels of conventional biomarkers were only elevated for a few days or did not change during the study period. Conclusions: The SP-D level is the most useful marker of the severity of lung injuries. These results suggest that the measurement of SP-D levels is an additional tool for monitoring acute lung injuries in rats.