The gradostat: a bidirectional compound chemostat and its application in microbiological research.
The gradostat: a bidirectional compound chemostat and its application in microbiological research.
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Gradostat：一种双向复合恒化器及其在微生物研究中的应用。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
J. Wimpenny
中科院分区：
文献类型：
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作者：
R. Lovitt;J. Wimpenny
描述了一种多阶段连续培养系统，其中溶质在容器之间以相反方向同时转移。该系统，称为梯度仪，产生相反的溶质梯度，是一个很好的实验室模型，许多自然的微生物生态系统中，溶质梯度是重要的。在稳态和非稳态条件下，使用有色染料的溶质转移的理论预测进行了确认。副球菌厌氧生长在梯度恒定器之间的交叉处的相反梯度的琥珀酸盐和硝酸盐。相反梯度的葡萄糖和氧气分离的芽孢杆菌（兼性厌氧菌）和丁酸梭菌（专性厌氧菌）的生长。这两个物种的活菌计数呈指数下降远离他们的生长位置在gradostat的两端。的gradostat和可能的替代构象的潜在价值进行了讨论。
A multistage continuous culture system is described in which solutes are transferred between vessels in opposite directions simultaneously. The system, called a gradostat, produces opposing solute gradients and is a good laboratory model of many natural microbial ecosystems in which solute gradients are important. Theoretical predictions concerning solute transfer were confirmed under steady-state and non steady-state conditions, using a coloured dye. Paracoccus denitrificans grew anaerobically in the gradostat at the intersection between opposing gradients of succinate and nitrate. Opposing gradients of glucose and oxygen separated the growth of a Bacillus sp. (a facultative anaerobe) and Clostridium butyricum (an obligate anaerobe). Viable counts for both species fell exponentially away from their growth positions at the ends of the gradostat. The potential value of the gradostat and possible alternative conformations are discussed.