Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications
Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications
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DOI：
10.1098/rsif.2005.0065
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发表时间：
2005-12-22
影响因子：
3.9
通讯作者：
Liu, KK
中科院分区：
文献类型：
--
作者：
Ahearne, M;Yang, Y;Liu, KK
我们提出了一种新的压痕方法来表征海藻酸盐和琼脂糖水凝胶构建物的粘弹性性质，这两种构建物通常被用作软生物组织的模型系统。利用灵敏的长工作距离显微镜测量了圆形水凝胶膜在恒定载荷作用下随时间变化的变形。侧向测量构造物的变形。根据基于Mooney-Rivlin弹性的大变形理论，可以确定作为时间函数的弹性模数。粘弹性理论，Zener模型，被用来关联不同凝胶浓度的构件的随时间变化的变形，因此可以定量地估计蠕变参数。杨氏模量值与凝胶浓度成正比。这一发现与其他出版物一致。我们的结果还表明，使用该技术测量含有合并的角膜基质细胞的凝胶的能力很强。这项研究展示了一种新的、方便的、无损、在线和实时测量水凝胶力学性能的技术。因此，这种新技术可以成为软组织工程的一种有价值的工具。
We present a novel indentation method for characterizing the viscoelastic properties of alginate and agarose hydrogel based constructs, which are often used as a model system of soft biological tissues. A sensitive long working distance microscope was used for measuring the time-dependent deformation of the thin circular hydrogel membranes under a constant load. The deformation of the constructs was measured laterally. The elastic modulus as a function of time can be determined by a large deformation theory based on Mooney-Rivlin elasticity. A viscoelastic theory, Zener model, was applied to correlate the time-dependent deformation of the constructs with various gel concentrations, and the creep parameters can therefore be quantitatively estimated. The value of Young's modulus was shown to increase in proportion with gel concentration. This finding is consistent with other publications. Our results also showed the great capability of using the technique to measure gels with incorporated corneal stromal cells. This study demonstrates a novel and convenient technique to measure mechanical properties of hydrogel in a non-destructive, online and real-time fashion. Thus this novel technique can become a valuable tool for soft tissue engineering.