An Integrative Analysis of Transcriptome, Proteome and Hormones Reveals Key Differentially Expressed Genes and Metabolic Pathways Involved in Flower Development in Loquat
An Integrative Analysis of Transcriptome, Proteome and Hormones Reveals Key Differentially Expressed Genes and Metabolic Pathways Involved in Flower Development in Loquat
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DOI：
10.3390/ijms21145107
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发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Liang, Guolu
中科院分区：
文献类型：
--
作者：
Jing, Danlong;Chen, Weiwei;Liang, Guolu
花的发育是多年生木本植物特别是果树生活史中的重要发育过程。本文采用转录组学、蛋白质组学和激素分析方法，对枇杷花芽分化、花芽伸长和开花期的关键候选基因/蛋白质进行了研究。比较转录组分析表明，差异表达基因主要集中在激素信号转导和淀粉蔗糖代谢途径。重要的是，激素信号转导的DEG显着参与生长素，赤霉素（GAs），细胞分裂素，乙烯，脱落酸（阿坝），茉莉酸和水杨酸的信号转导途径。与此同时，关键的花整合基因FLOWERLOCUS T（FT）和SUPPER OF OVEREXPRESSION OF CONSTANS 1（SOC 1）以及花分生组织身份基因SPL、LEAFY（LFY）、APETALA 1（AP 1）和AP 2在FBD期显著上调。然而，关键的花器官身份基因AGAMOUS（AG），AP 3和PISTILLATA（PI）在FBE和FA阶段显著上调。此外，转录因子如bHLH（basic helix-loop-helix）、NAC（no apical meristem）、拟南芥转录激活因子（ATAF 1/2）和杯状子叶（CUC 2）、MYB_related（myeloblastosis_related）、ERF（ethylene response factor）和C2 H2（cysteine-2/histidine-2）也有显著差异表达。因此，差异积累的蛋白质（DAP）和DEG和DAP的组合富集的比较蛋白质组学分析表明，淀粉和蔗糖代谢也显着富集。GA（3）和玉米素的浓度在FA期前较高，而阿坝的浓度在FA期仍较高。本研究结果为揭示枇杷花发育的内在机制提供了丰富的序列资源。
Flower development is a vital developmental process in the life cycle of woody perennials, especially fruit trees. Herein, we used transcriptomic, proteomic, and hormone analyses to investigate the key candidate genes/proteins in loquat (Eriobotrya japonica) at the stages of flower bud differentiation (FBD), floral bud elongation (FBE), and floral anthesis (FA). Comparative transcriptome analysis showed that differentially expressed genes (DEGs) were mainly enriched in metabolic pathways of hormone signal transduction and starch and sucrose metabolism. Importantly, the DEGs of hormone signal transduction were significantly involved in the signaling pathways of auxin, gibberellins (GAs), cytokinin, ethylene, abscisic acid (ABA), jasmonic acid, and salicylic acid. Meanwhile, key floral integrator genesFLOWERING LOCUS T(FT) andSUPPRESSOR OF OVEREXPRESSION OF CONSTANS1(SOC1) and floral meristem identity genesSQUAMOSA PROMOTER BINDING LIKE(SPL),LEAFY(LFY),APETALA1(AP1), andAP2were significantly upregulated at the FBD stage. However, key floral organ identity genesAGAMOUS(AG),AP3, andPISTILLATA(PI) were significantly upregulated at the stages of FBE and FA. Furthermore, transcription factors (TFs) such asbHLH(basic helix-loop-helix),NAC(no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF1/2) and cup-shaped cotyledon (CUC2)),MYB_related(myeloblastosis_related),ERF(ethylene response factor), andC2H2(cysteine-2/histidine-2) were also significantly differentially expressed. Accordingly, comparative proteomic analysis of differentially accumulated proteins (DAPs) and combined enrichment of DEGs and DAPs showed that starch and sucrose metabolism was also significantly enriched. Concentrations of GA(3)and zeatin were high before the FA stage, but ABA concentration remained high at the FA stage. Our results provide abundant sequence resources for clarifying the underlying mechanisms of the flower development in loquat.