The Expression Pattern of ArgAH1 and ArgAH2 Genes in Arabidopsis thaliana
The Expression Pattern of ArgAH1 and ArgAH2 Genes in Arabidopsis thaliana
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ArgAH1和ArgAH2基因在拟南芥中的表达模式
DOI：
10.5376/gab.2018.09.0003
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
卜媛媛
中科院分区：
文献类型：
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作者：
卜媛媛
尿素是农业生产中氮素的主要形态，也是植物体内重要的氮代谢产物。在盐胁迫下，发芽种子中的精氨酸可以通过精氨酸酶降解精氨酸而产生尿素。为了更好地了解Arginase基因的生物学功能，本研究对ArgAH1和ArgAH2基因在盐胁迫和不同氮源胁迫下的表达进行了分析。我们的研究结果表明，在正常条件下，ArgAH1和ArgAH2分别在拟南芥的根和角果中高表达。然而，ArgAH1和ArgAH2的表达水平上调盐胁迫在根和子叶。此外，ArgAH1和ArgAH2的表达水平也对不同的氮源（如NH4Cl、尿素、精氨酸）有反应，在这些处理条件下，根和子叶中的表达模式也不同。这些结果表明，这两个转肽酶基因在植物响应非生物胁迫中具有不同的生物学功能。
Urea is the major nitrogen form supplied as fertilizer in agricultural production and also an important nitrogen metabolite in plants. Urea can be generated from Arginine breakdown by Arginase in germinating seeds under salt stress. To better understanding the biological functions of Arginase gene, here, we carried out the expression analysis of ArgAH1 and ArgAH2 genes in response to salt stress and different nitrogen source during early seed germination and seedling growth stage. Our results show that ArgAH1 and ArgAH2 were highly expressed in root and siliques of Arabidopsis under normal conditions, respectively. However, the expression levels of ArgAH1 and ArgAH2 was up-regulated by salt stress in both roots and cotyledons. In addition, the expression levels of ArgAH1 and ArgAH2 were also found to response to different nitrogen sources (e.g. NH 4 Cl, urea, arginine), different expression pattern are shown in both the roots and cotyledons under these treatment conditions. These results suggest that two arginase genes have different biological function in response to abiotic stresses in plants.