Inhibited differentiation of Langerhans cells in the rat epidermis upon systemic treatment with cyclosporin A
Inhibited differentiation of Langerhans cells in the rat epidermis upon systemic treatment with cyclosporin A
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DOI：
10.14670/hh-16.107
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Filipponi, F
中科院分区：
文献类型：
--
作者：
Borghi-Cirri, MB;Riccardi-Arbi, R;Filipponi, F
已知免疫抑制剂环孢素A （CsA）可导致朗格汉斯细胞（LC）数量、DNA合成和功能的减少。由于已知LC的分化在获得性免疫缺陷而非药物的情况下受到阻碍，我们研究了CsA是否会发生这种情况。大鼠皮下注射CsA（5、10、50 mg.kg(-1)）。D(-1))三周；采用免疫组化和电镜对皮肤进行分析。在未处理的对照组中，表皮免疫标记细胞每100个基底角质形成细胞为15+/-3.5（平均+/- SEM），增加CsA剂量后为8.75+/-1.3、4.75+/-1.0和1.7+/-1.2
The immunosuppressant drug cyclosporin A (CsA) is known to cause reduction in number, DNA synthesis and function of Langerhans cells (LC). Since also the differentiation of LC is known to be hampered in conditions of acquired immunodeficiency not due to drugs, we investigated whether this occurs with CsA. Rats were injected subcutaneously with CsA (5, 10 and 50 mg.kg(-1).d(-1)) for three weeks; the skin was analyzed by Ia immunohistochemistry and by electron microscopy. Epidermal immunolabeled cells were 15+/-3.5 (mean +/- SEM) per 100 basal keratinocytes in untreated controls and 8.75+/-1.3, 4.75+/-1.0 and 1.7+/-1.2 upon increasing doses of CsA (p