The alpha beta T cell receptor can replace the gamma delta receptor in the development of gamma delta lineage cells
The alpha beta T cell receptor can replace the gamma delta receptor in the development of gamma delta lineage cells
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DOI：
10.1016/s1074-7613(00)80260-5
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发表时间：
1996-10-01
期刊：
影响因子：
32.4
通讯作者：
vonBoehmer, H
中科院分区：
文献类型：
--
作者：
Bruno, L;Fehling, HJ;vonBoehmer, H
在表达由转基因TCR识别的标称抗原（HY肽加H-2D（B）MHC）的TCR转基因小鼠的外周淋巴组织中，存在携带转基因TCR的不寻常的CD 4（-）CD 8（-）和CD 4（-）CD 8（低）细胞。在这里，我们表明，不像TCR α β T细胞产生的名义抗原的情况下，这些不寻常的细胞不表达内源性TCR α基因，保持了两个染色体上的TCR δ基因座，并可以共表达TCR α β和TCR γ δ链上的细胞表面。后者也适用于雄性和雌性TCR转基因小鼠中的CD 4（-）CD 8（-）、HSA（+）TCR α β（+）胸腺细胞。TCR α β和TCR γ δ共表达细胞的数量在前TCR缺陷小鼠中增加。数据表明，在γ δ谱系细胞的发育中，TCR α β可以取代TCR γ δ，并且前TCR干扰γ δ表达细胞的产生。
In peripheral lymphoid tissues of TCR transgenic mice that express the nominal antigen (HY peptide plus H-2D(b) MHC) recognized by the transgenic TCR, there exist unusual CD4(-)CD8(-) and CD4(-)CD8(low) cells bearing the transgenic TCR. Here we show that, unlike TCR alpha beta T cells that are generated in the absence of nominal antigen, these unusual cells do not express endogenous TCR alpha genes, have maintained the TCR delta locus on both chromosomes, and can coexpress TCR alpha beta and TCR gamma delta chains on the cell surface. The latter is also true for CD4(-)CD8(-), HSA(+) TCR alpha beta(+) thymocytes in male and female TCR transgenic mice. The number of TCR alpha beta and TCR gamma delta coexpressing cells is increased in pre-TCR-deficient mice. The data indicate that the TCR alpha beta can replace the TCR gamma delta in the development of gamma delta lineage cells and that the pre-TCR interferes with the generation of gamma delta-expressing cells.